An Aptamer-based Colorimetric Sensor for Streptomycin and Its Application in Food Inspection

doi:10.1007/s40242-017-7029-6

Chemical Research in Chinese Universities ›› 2017, Vol. 33 ›› Issue (5): 714-720.doi: 10.1007/s40242-017-7029-6

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An Aptamer-based Colorimetric Sensor for Streptomycin and Its Application in Food Inspection

LIU Zhongcheng^1,2, ZHANG Yanfen³, XIE Yao², SUN Ying³, BI Kewei², CUI Zhe², ZHAO Lijian², FAN Wufang¹

1. College of Life Sciences, Hebei University, Baoding 071002, P. R. China;
2. Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Baoding 071002, P. R. China;
3. Office of Science and Technology, Hebei University, Baoding 071002, P. R. China

Received:2017-01-16 Revised:2017-06-23 Online:2017-10-01 Published:2017-08-07
Contact: FAN Wufang,E-mail:wfan@hbu.edu.cn E-mail:wfan@hbu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.81202338);the Natural Science Foundation of Hebei Province, China(No.H2016201121);the National Training Program of Innovation and Entrepreneurship for Undergraduates,China(No.201610075001); the Postdoctoral Science Foundation of China(No.2013M530885);the Science and Technology Research Project of Hebei Higher Education Institutions, China(No.ZD2017010).

Abstract

Abstract:

An aptamer-based colorimetric biosensor was developed,which could be used to detect residual streptomycin from food quickly and cost-effectively.The ssDNA aptamer target for streptomycin was obtained using systemic evolution of ligands by exponential enrichment through affinity chromatography.A total of 19 candidates were obtained after 10 rounds of selection and were grouped into 3 families according to their similarity of sequence and structure.Among them,aptamer A15 showed the highest affinity for streptomycin tested by fluorescence intensity analysis with a dissociation constant of 6.07 nmol/L.Aptamer A15 also exhibited a higher streptomycin specificity with the lowest detectable limit of 25 nmol/L according to the value of A₆₂₀/A₅₂₀ ratio with AuNP-based colorimetric assay.The method was specific and sensitive for the detection of streptomycin from simply-treated milk and honey at 100 and 125 nmol/L,respectively,and is a promising approach to monitor antibiotics or other small molecules analogues in food inspection.

Key words: Aptamer, Streptomycin, Systematic evolution of ligands by exponential enrichment(SELEX), Au nanoparticle, Aptasensor

LIU Zhongcheng, ZHANG Yanfen, XIE Yao, SUN Ying, BI Kewei, CUI Zhe, ZHAO Lijian, FAN Wufang. An Aptamer-based Colorimetric Sensor for Streptomycin and Its Application in Food Inspection[J]. Chemical Research in Chinese Universities, 2017, 33(5): 714-720.

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An Aptamer-based Colorimetric Sensor for Streptomycin and Its Application in Food Inspection

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