Simultaneous Determination of Catecholamines and Related Metabolites by Capillary Electrophoresis with Amperometric Detection

doi:10.1007/s40242-013-3132-5

Chemical Research in Chinese Universities ›› 2013, Vol. 29 ›› Issue (5): 850-853.doi: 10.1007/s40242-013-3132-5

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Simultaneous Determination of Catecholamines and Related Metabolites by Capillary Electrophoresis with Amperometric Detection

ZHANG Hai-tao¹, LI Zhuo¹, ZHANG Jun-bo¹, ZHANG Yang¹, YE Jian-nong¹, CHU Qing-cui¹, ZHANG Mei-jiang²

1. Department of Chemistry, East China Normal University, Shanghai 200241, P. R. China;
2. Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, School of Sports and Health Care, East China Normal University, Shanghai 200062, P. R. China

Received:2013-03-20 Revised:2013-06-07 Online:2013-10-01 Published:2013-09-17
Contact: CHU Qing-cui, ZHANG Mei-jiang E-mail:qcchu@chem.ecnu.edu.cn;mjzhang@tyxx.ecnu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.21205042) and the Special Funds for the Development of Major Scientific Instruments and Equipment, China(No.2011YQ15007205).

Abstract

Abstract:

An electrophoretic method was developed for the determination of several important catecholamine markers, namely norepinephrine, epinephrine, dopamine, metanephrine, vanillylmandelic acid and homovanillic acid in urine samples. Under the optimum conditions, the six marker compounds could be well separated with the major coexisting interference compound uric acid within 24 min at a separation voltage of 16 kV in a borate running buffer (80 mmol/L, pH=9.48). Highly linear response can be obtained over three orders of magnitude for the above markers with the low limits of detection ranging from 1.0 ng/mL to 5.0 ng/mL(S/N=3). The proposed capillary electrophoresis with amperometric detection(CE-AD) method has been used to simultaneously determine the six catecholamine markers in urine samples of healthy volunteers and patients suffering from different diseases avoiding redundant measurements and high assay cost; and the electrochemical profiles can suggest more diagnostic information of multiple diseases, which provides a promising and convenient entry into primary diagnoses of several clinical diseases.

Key words: Amperometric detection, Capillary electrophoresis, Catecholamine, Metabolic marker, Urine

ZHANG Hai-tao, LI Zhuo, ZHANG Jun-bo, ZHANG Yang, YE Jian-nong, CHU Qing-cui, ZHANG Mei-jiang. Simultaneous Determination of Catecholamines and Related Metabolites by Capillary Electrophoresis with Amperometric Detection[J]. Chemical Research in Chinese Universities, 2013, 29(5): 850-853.

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Simultaneous Determination of Catecholamines and Related Metabolites by Capillary Electrophoresis with Amperometric Detection

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