Chemical Research in Chinese Universities ›› 2023, Vol. 39 ›› Issue (2): 253-259.doi: 10.1007/s40242-022-2165-z

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Preparation of Cerium Dioxide Functionalized Magnetic Layered Double Hydroxides for High-efficiency Phosphopeptide Enrichment

JIANG Dandan1, QI Ruixue1, LYU Siqi1, WANG Wei1, LIU Jinghai1, JIA Qiong2   

  1. 1. College of Chemistry and Materials Science, Inner Mongolia Key Laboratory of Carbon Nanomaterials, Inner Mongolia Minzu University, Tongliao 028000, P. R. China;
    2. College of Chemistry, Jilin University, Changchun 130012, P. R. China
  • Received:2022-05-18 Online:2023-04-01 Published:2023-03-16
  • Contact: LIU Jinghai, JIA Qiong E-mail:jhliu2008@sinano.ac.cn;jiaqiong@jlu.edu.cn
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (Nos.22004073 and 21961024), the Natural Science Foundation of Inner Mongolia, China(No.2020BS02010), the Talent Research Support Funds from Government-Sponsored Institution of Inner Mongolia, China(No.RCQD19002), the Inner Mongolia Minzu University Doctoral Research Startup Fund Project, China(No.BS516), and the Incentive Funding from Nano Innovation Institute(NII) of Inner Mongolia Minzu University, China.

Abstract: In this work, we prepared a material with magnetic nanoparticles (Fe3O4) as core, layered double hydroxides(LDHs) as affinity shell, and cerium dioxide(CeO2) as functional molecules(denoted as Fe3O4@LDH-CeO2). On the basis of combined immobilized metal ion affinity chromatography(IMAC) and metal oxide affinity chromatography(MOAC), Fe3O4@LDH-CeO2 was used to enrich phosphopeptides with high efficiency. The material exhibited high selectivity(α-casein:β-casein:BSA=1:1:5000, mass ratio), high recovery(95.87%), and good reusability of 10 times adsorption- desorption experiments. The feasibility of Fe3O4@LDH-CeO2 was further investigated by extracting phosphopeptides from biological samples(nonfat milk, serum, saliva, and A549 cell lysate).

Key words: Layered double hydroxide, Cerium dioxide, Magnetic, Phosphopeptide enrichment