Influences of Synthesis Conditions on the Formation of Methotrexate Intercalated Layered Double Hydroxides by Exfoliation-reassembly Route

doi:10.1007/s40242-019-8371-7

Abstract

Abstract: The intercalation of methotrexate(MTX) into layered double hydroxides(LDHs) via an exfoliation-reassembly process was performed and the resulting hybrids were then characterized by X-ray diffractiometry(XRD), Fourier transform infrared(FTIR) spectroscopy, thermogravimetry(TG) and differential scanning calorimetry(DSC), etc. In the synthesis procedure, LDHs particles were firstly delaminated, and the reassembly process was performed to get MTX intercalated LDH(MTX/LDHs) hybrids. The reassembly conditions, such as reassembly time and different reassembly solvent, were studied emphatically. It was found that 20 min is the optimum time and formamide is the most suitable solvent in the reassembly process. At last, the controlled release property and the drug-loading capacity were examined emphatically to reveal that MTX/LDHs hybrids reassembled in formamide exhibit the highest drug-loading capacity. The cytotoxicity of the hybrids was estimated by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide,thiazolyl blue tetrazolium bromide(MTT) assays on the human lung cancer cells(A549), with the results showing that MTX/LDHs hybrids have effective suppress role on the proliferation of cancer cells, and the hybrids obtained from the formamide solution present much better anticancer effect than the other samples.

Key words: Methotrexatum, Layered double hydroxide, Exfoliation-reassembly process

LIU Zhenlei, JIA Qingyang, ZHAO Xiufen, LI Shuping, SHEN Jian, LI Xiaodong. Influences of Synthesis Conditions on the Formation of Methotrexate Intercalated Layered Double Hydroxides by Exfoliation-reassembly Route[J]. Chemical Research in Chinese Universities, 2019, 35(5): 901-907.

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