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Chemical Research in Chinese Universities ›› 2006, Vol. 22 ›› Issue (5): 598-601.doi:

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Studies on Fragmentation Pathways of N-Ethoxy(phenyl) phosphoryl Amino Acids by Electrospray Ionization Tandem Mass Spectrometry

CAO Shu-xia1, LI Hong-wei1, GUO Yan-chun1, LIAO Xin-cheng1 and ZHAO Yu-fen1,2
   

    1. The Key Laboratory of Chemical Biology and Organic Chemistry, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, P. R. China;
    2. The Key Laboratory for Bioorganic Phosphorus Chemistry and Chemical Biology, Ministry of Education, Department of Chemistry, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084, P. R. China
  • Received:2005-08-24 Revised:1900-01-01 Online:2006-09-25 Published:2006-09-25
  • Contact: ZHAO Yu-fen E-mail:csx@zzu.edu.cn

Abstract: The positive and negative ESI-MS/MS spectra of N-ethoxy(phenyl) phosphoryl amino acids(EPP-AA) were investigated by electrospray ionization(ESI) ion trap mass spectrometry. The fragmentation pathways of [M+Na]+ and [M-H]- ions are proposed and rationalized. The observation may have some potential applications in the interpretation of the MS/MS spectra of novel N-phosphoryl compounds. The complexity of MS/MS spectra of EPP-AA [M+Na]+ ions is decreased compared with that of N-dialkyloxyphosphoryl amino acid. Therefore, the new phosphonamidate method may be considered one of the superior methods that can be used in sequencing peptides and proteins extensively.

Key words: N-Ethoxy(phenyl)phosphoryl amino acid, Electrospray ionization ion trap mass spectrometry, Tandem mass spectrometry, N-Ethoxy(phenyl) phosphoryl amino acid, Electrospray ionization ion trap mass spectrometry, Tandem mass spectrometry