Delivery of Survivin siRNA Using Cationic Diphenylalanine Vesicles

doi:10.1007/s40242-019-8184-8

Chemical Research in Chinese Universities ›› 2019, Vol. 35 ›› Issue (3): 434-439.doi: 10.1007/s40242-019-8184-8

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Delivery of Survivin siRNA Using Cationic Diphenylalanine Vesicles

GUAN Shuwen¹, YU Xiaoxuan¹, LI Junyang¹, XU Heng², HAN Wenzhao¹, SHI Guannan¹, XU Jia¹, WANG Liping¹

1. School of Life Sciences, Jilin University, Changchun 130012, P. R. China;
2. College of Chemistry, Jilin University, Changchun 130012, P. R. China

Received:2018-05-31 Revised:2019-04-04 Online:2019-06-01 Published:2019-05-29
Contact: WANG Liping E-mail:wanglp@jlu.edu.cn
Supported by:
Supported by the Science and Technology Development Program of Jilin Province, China(No.20150520157JH).

Abstract

Abstract: Small interfering RNA(siRNA) has been proved to be a powerful tool for silencing target gene in cells, raising the possibility that siRNA can be employed as a therapy for treating cancers and other genetic diseases. However, siRNA transfection has the limitation due to the difficulty in the delivery of siRNA to target cells and tissues. To explore an efficient biocompatible siRNA delivery system, cationic diphenylalanine vesicles(CDPVs) were constructed to transfer survivin siRNA to human ovarian cancer cells. The morphology of CDPVs was characterized by scanning electron microscopy(SEM) and the distribution of survivin siRNA was characterized by confocal laser scanning microscopy, which reveal that diphenylalanine and the survivin siRNA were successfully co-delivered. After co-incubation for 48 h, the CDPVs/siRNA exhibited enhanced tumor cell growth inhabitation and apoptosis inducted in human SK-OV-3 ovarian carcinoma cells. Overall, CDPVs is an efficient siRNA delivery system and has a pro- mising prospect for cancer therapy.

Key words: siRNA delivery, Cationic diphenylalanine nanovesicle, Cancer therapy

GUAN Shuwen, YU Xiaoxuan, LI Junyang, XU Heng, HAN Wenzhao, SHI Guannan, XU Jia, WANG Liping. Delivery of Survivin siRNA Using Cationic Diphenylalanine Vesicles[J]. Chemical Research in Chinese Universities, 2019, 35(3): 434-439.

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Delivery of Survivin siRNA Using Cationic Diphenylalanine Vesicles

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