Improving Energy Metabolism of Deproteinized Extract of Calf Blood Through Regulation of Hmgcs2, Cpt1a, Angptl4, Cyp8b1, and Ehhadh Genes in Mice

doi:10.1007/s40242-019-9021-9

Chemical Research in Chinese Universities ›› 2019, Vol. 35 ›› Issue (3): 427-433.doi: 10.1007/s40242-019-9021-9

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Improving Energy Metabolism of Deproteinized Extract of Calf Blood Through Regulation of Hmgcs2, Cpt1a, Angptl4, Cyp8b1, and Ehhadh Genes in Mice

ZHOU Tong¹, XU Guangyu², SUN Luyao³, YU Zhenxiang⁴, WANG Guixia¹

1. Department of Endocrinology and Metabolism, the First Hospital of Jilin University, Changchun 130021, P. R. China;
2. College of Pharmacy, Beihua University, Jilin 132013, P. R. China;
3. Department of Infectious Diseases, the First Hospital of Jilin University, Changchun 130021, P. R. China;
4. Department of Respiration, the First Hospital of Jilin University, Changchun 130021, P. R. China

Received:2019-01-21 Revised:2019-04-16 Online:2019-06-01 Published:2019-05-29
Contact: YU Zhenxiang, WANG Guixia E-mail:yuzhenxiang2005@sina.com;gwang168@jlu.edu.cn
Supported by:
Supported by the Natural Science Foundation of Jilin Province, China(No.20180101099JC) and the "Thirteen Five" Science and Technology Project of the Department of Education of Jilin Province, China(No.JJKH20180372KJ).

Abstract

Abstract: Herein, we described the physicochemical properties of deproteinized extract of calf blood(DECB) and established a hypoxia model treated with or without DECB to detect the sugar, lactic acid, protein, and ATP contents of mice and then identified and analyzed the differentially expressed genes between two groups using mRNA expression chip. According to the results of the airtight hypoxia experiment, mice in the model+DECB group had a significantly prolonged time of hypoxia tolerance compared with the model group. The biochemical test indicated that DECB could significantly increase the level of sugar, ATP and proteins and reduce the amount of lactic acid in mice. It also revealed that Hmgcs2, Cpt1a, Angptl4, Cyp8b1, and Ehhadh genes were involved in mice energy metabolism, and were closely associated with metabolic signaling pathway. These results suggest that DECB might be a potential drug to treat metabolic diseases. Among the genes with differential expression under hypoxia, Angptl4, Cyp8b1, and Ehhadh were critical factors for sugar metabolism. Hmgcs2 provided energy directly, and Cptla regulated cellular inflammatory responses, promoting energy metabolism.

Key words: Deproteinized extract of calf blood, Energy metabolism, mRNA chip

ZHOU Tong, XU Guangyu, SUN Luyao, YU Zhenxiang, WANG Guixia. Improving Energy Metabolism of Deproteinized Extract of Calf Blood Through Regulation of Hmgcs2, Cpt1a, Angptl4, Cyp8b1, and Ehhadh Genes in Mice[J]. Chemical Research in Chinese Universities, 2019, 35(3): 427-433.

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Improving Energy Metabolism of Deproteinized Extract of Calf Blood Through Regulation of Hmgcs2, Cpt1a, Angptl4, Cyp8b1, and Ehhadh Genes in Mice

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