CRCU

Chemical Research in Chinese Universities ›› 2012, Vol. 28 ›› Issue (5): 792-796 .

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Detection of Penicillin via Surface Plasmon Resonance Biosensor

ZHANG Ying, JIN Wei, YANG Meng-chao, ZHANG Ti-qiang, ZHOU Chao, XIE Fei, SONG Qi, REN Hao, JIN Qin-han, MU Ying   

  1. Research Center for Analytical Instrumentation, Institute of Cyber-systems and Control, State Key Laboratory of Industrial Control Technology, Department of Control Science and Technology, Zhejiang University, Hangzhou 310058, P. R. China
  • Received:2012-03-19 Revised:2012-05-22 Online:2012-09-25 Published:2012-09-07
  • Supported by:
    Supported by the National Natural Science Foundation of China(No.31070772), the Science and Technology Programs of Zhejiang Province, China(No.2011C37029), the Doctoral Program of Higher Education of China(No.200901011110136) and the Science and Technology Programs of Suzhou City, China(No.ZXG0920).

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Abstract: A method of using Au colloid to capture the decomposed product of penicillin, penicillamine, on a surface plasmon resonance(SPR) biosensor for the quantitative determination of penicillin was developed. Based on the decomposition of penicillin to generate penicillamine and penilloaldehyde, a high sensitive biosensor for detecting penicillin was also developed. In our experiment, it was penicillamine rather than penicillin that has been measured. This is because penicillamine contains a functional group that makes it self-assembling on Au colloid to increase the molecular weight so as to improve the surface plasmon resonance signal. On a UV-Vis spectrophotometer, a high concentration of penicilliamine-Au complex was determined, indicating that penicillamine was already well combined with Au colloid. The method, using the combination of Au colloid with penicillamine, proved to detect penicillin.

Key words: Surface plasmon resonance(SPR), Biosensor, Penicillamine, Au colloid