CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (6): 934-938.

• Articles • Previous Articles     Next Articles

Electrochemical Determination of Reduced Glutathione at Multiwalled Carbon Nanotubes/Poly(bromocresol green) Modified Glassy Carbon Electrode

HOU Ju-ying and AI Shi-yun*   

  1. College of Chemistry and Material Science, Shandong Agricultural University, Tai'an 271018, P. R. China
  • Received:2010-12-30 Revised:2011-05-26 Online:2011-11-25 Published:2011-11-07
  • Contact: AI Shi-yun E-mail:ashy@sdau.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.20775044) and the Natural Science Foundation of Shandong Province of China(No.Y2006B20).

PDF (PC)

Cited

5

Abstract: A multiwalled carbon nanotubes/poly(bromocresol green) modfied glassy carbon electrode (MWNTs-PBG/GCE) was used to investigate the electrochemical behavior of reduced glutathione(GSH). GSH showed an irreversible oxidation process on MWNTs-PBG/GCE with an oxidation peak at 0.77 V(vs. SCE) in a phosphate buffer solution(pH=4.0). The kinetic parameters of the electrochemical behavior of GSH on MWNTs-PBG/GCE were calculated. Under the optimal conditions and with the help of amperometric method, a linear relationship was obtained between the oxidation peak current and GSH concentration in the range from 2×10–7 mol/L to 5×10–6 mol/L with the detection limit as 1×10–8 mol/L(signal-to-noise ratio of 3). The current reached the steady-state current within about 5 s. The modified electrode surface had very good reproducibility and stability.

Key words: Poly(bromocresol green), Glutathione, Electrode, Multiwalled carbon nanotube