CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (4): 570-573.

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Raman and UV-Vis Spectroscopy Applied to the Analysis of Liver Tissues from Rats with Myocardial Ischemia Induced by Isoproterenol

GAO Hai-cheng1, ZOU Ying-gang2, HUANG Yu-xin3, GAO Hai-mei2, CHEN Lei4 and PEI Jin1*   

  1. 1. School of Pharmaceutical Sciences, Jilin University, Changchun 130012, P. R. China;
    2. Department of Obstetrics & Gynecology, the Second Hospital of Jilin University, Changchun 130041, P. R. China;
    3. Basic Science College, Changchun University of Technology, Changchun 130012, P. R. China;
    4. Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun 130012, P. R. China
  • Received:2010-08-31 Revised:2010-12-22 Online:2011-07-25 Published:2011-06-29
  • Contact: PEI Jin E-mail:peijin@jlu.edu.cn
  • Supported by:

    Supported by the Scientific and Technological Developing Scheme of Jilin Province, China(No.201101067).

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Abstract: The application of the laser Raman spectroscopic(LRS) technique for the analysis of liver tissues from rats with myocardial ischemia induced by isoproterenol(ISO) was described. Animal model of myocardial ischemia was established for rats induced by ISO. Rats were randomly divided into four groups as normal group and myocardial ischemia groups. We observed the successful myocardial ischemia model via serum enzymes levels and hematoxylin-eosin(HE) staining, and detected the liver tissue of the rats from normal group and liver tissue of the rats from myocardial ischemia groups via UV-Vis spectroscopy(UV-Vis) and LRS, and the changes of the absorbance spectra were compared in the above four different groups. The results show that ISO can induce rat myocardial ischemia successfully. The spectrum of normal liver tissue supernatant exhibits a strong absorption band at 968 nm，but no absorption band appears in the spectra of liver tissue supernatant solutions from the rats with myocardial ischemia induction after 2, 12 and 72 h presented at 968 nm. LRS results show that Raman intensities of the precipitates suffered from ISO-treatment after 2, 12 and 72 h were obviously increased compared with that of the precipitate of the liver tissue of the normal rats suffered from 0.9 g/L normal saline(NS) treatment. These results indicate that LRS and UV-Vis can be harmless, nondestructive, rapid and effective methods for analyzing different pathological specimens of liver tissue from myocardial ischemia rats.

Key words: Laser Raman spectroscopy, UV-Vis spectroscopy, Isoproterenol, Myocardial ischemia, Rat, Liver tissue