Enantiomeric Separation of Antidepressant Trimipramine by Capillary Electrophoresis Combined with Electrochemiluminescence Detection in Aqueous-organic Media

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (1): 34-37.

• Articles • Previous Articles Next Articles

Enantiomeric Separation of Antidepressant Trimipramine by Capillary Electrophoresis Combined with Electrochemiluminescence Detection in Aqueous-organic Media

YU Cai-xia^1,2, YUAN Bai-qing¹ and YOU Tian-yan^1*

1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
2. Graduate School of Chinese Academy of Sciences, Beijing 100049, P. R. China

Received:2010-02-09 Revised:2010-04-28 Online:2011-01-25 Published:2011-01-04
Contact: YOU Tian-yan E-mail:youty@ciac.jl.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.20875085) and the Fund of Chinese Academy of Sciences(No.KJCX2-YW-H11).

Abstract

Abstract: The antidepressant trimipramine(Tri) enantiomers were successfully separated by capillary electrophoresis(CE) coupled with electrochemiluminescence(ECL) detection in aqueous-organic media. A dual cyclodextrin(CD) system combining β-CD and hydroxypropyl-β-cyclodextrin(HP-β-CD) was used as chiral selector. Acetonitrile(ACN) was added to the running buffer to improve the separation efficiency, detection sensitivity and repeatability. The method was also successfully applied to the chiral separation of Tri in spiked human urine sample.

Key words: Capillary electrophoresis, Electrochemiluminescence, Trimipramine, Enantioseparation, Organic additive

YU Cai-xia, YUAN Bai-qing and YOU Tian-yan*. Enantiomeric Separation of Antidepressant Trimipramine by Capillary Electrophoresis Combined with Electrochemiluminescence Detection in Aqueous-organic Media[J]. Chemical Research in Chinese Universities, 2011, 27(1): 34-37.

[1]	HU Shifan, WANG Yafeng and SU Bin. Effect of Ionic Strength on the Electrochemiluminescence Generation by Tris(2,2'-bipyridyl)ruthenium(II)/Tri-n-propylamine [J]. Chemical Research in Chinese Universities, 2022, 38(3): 816-822.
[2]	PENG Bo, FU Shiguo, LI Yanxia, ZHANG Junhui, XIE Shengming, LI Li, LYU Yun, DUAN Aihong, CHEN Xuexian, YUAN Liming. Enantioseparation by HPLC Using an Inorganic Chiral Mesoporous Silica with Highly-ordered Structure [J]. Chemical Research in Chinese Universities, 2019, 35(6): 978-982.
[3]	ZHANG Hai-tao, LI Zhuo, ZHANG Jun-bo, ZHANG Yang, YE Jian-nong, CHU Qing-cui, ZHANG Mei-jiang. Simultaneous Determination of Catecholamines and Related Metabolites by Capillary Electrophoresis with Amperometric Detection [J]. Chemical Research in Chinese Universities, 2013, 29(5): 850-853.
[4]	LIU Fan, ZHANG Ling-yi, QIAN Jun-hong, GAO Fang-yuan, REN Jun, ZHANG Wei-bing. Construction of Post-column Micro-membrane Reactor for Protein Analysis in Capillary Electrophoresis with Laser Induced Fluorescence Detection [J]. Chemical Research in Chinese Universities, 2013, 29(5): 828-830.
[5]	LI Xiao-tian, ZHAO Ya-jing, JIANG Cheng-fei, ZHANG Han-qi, YU Ai-min. Determination of Amino Acids in Panax notoginseng by Microwave Hydrolysis and Derivatization Coupled with Capillary Zone Electrophoresis Detection [J]. Chemical Research in Chinese Universities, 2013, 29(3): 434-438.
[6]	SUN Jin-ying, XU Xiao-yu, YU Huan, YOU Tian-yan. Determination of Nicotine in Tobacco by Capillary Electrophoresis with Electrochemical Detection [J]. Chemical Research in Chinese Universities, 2012, 28(3): 415-418.
[7]	ZHOU Xiao-guang, YUAN Bai-qing, ZHANG Lei, LIU Yang, HAN Dong-xue and YOU Yang. Determination of Amino Acids by Ru(bpy)₃²⁺ Cathodic Electrochemiluminescence with High Sensitivity [J]. Chemical Research in Chinese Universities, 2010, 26(3): 376-379.
[8]	SHI Hong-yu, ZHANG Hai-zhu, LONG Yuan-de and HUANG Tian-bao. Modification of Chiral Stationary Phases with L-Proline as a Selector for Ligand-exchange Chromatography via* Introducing Hydrophobic Groups [J]. Chemical Research in Chinese Universities, 2009, 25(6): 822-826.
[9]	ZHOU Xiao-guang , SUN Jin-ying , ZHU De-rong , YUAN Bai-qing , YOU Tian-yan . Quality Analysis of Herbal Medicine Products Prepared from Herba Sarcandrae by Capillary Electrophoresis with Electrochemical Detection [J]. Chemical Research in Chinese Universities, 2008, 24(2): 148-153.
[10]	XIANG Qian , NIU Gang , WU Xian-hua , CHEN Gang . Stability and Determination of Metamizole Sodium by Capillary Electrophoresis Analysis Combined with Infra-red Spectroscopy [J]. Chemical Research in Chinese Universities, 2007, 23(6): 654-658.
[11]	TIAN Yi-ling, CHEN Guan-hua. Determination of Magnolol and Honokiol by Non-aqueous Capillary Electrophoresis [J]. Chemical Research in Chinese Universities, 2006, 22(3): 335-338.
[12]	WANG Ying, MO Jin-yuan, CHEN Zuan-guang, GAO Yan. Subtracting Technique of Baselines for Capillary Electrophoresis Signals [J]. Chemical Research in Chinese Universities, 2004, 20(2): 134-137.
[13]	LI Ying-xiu, ZHU Lian-de, ZHU Guo-yi. A Novel Flow Injection Optical Fiber Biosensor for Hypoxanthine Based on Luminol Electrochemiluminescence [J]. Chemical Research in Chinese Universities, 2003, 19(2): 240-244.
[14]	DENG Guang-hui, WEI Shou-lian, MO Jin-yuan. Chiral Separation of Ibuprofen and Terbutaline by Nonaqueous Capillary Electrophoresis with Conductance Detection [J]. Chemical Research in Chinese Universities, 2002, 18(1): 16-19.
[15]	ZHANG Xin-xiang, HONG Feng, BAI Jing, CHANG Wen-bao, CI Yun-xiang . Preparation of β-Cyclodextrin Modified Fused Silica Capillary and Its Application in Capillary Electrophoresis Enantiomeric Separation [J]. Chemical Research in Chinese Universities, 1999, 15(3): 232-237.

Enantiomeric Separation of Antidepressant Trimipramine by Capillary Electrophoresis Combined with Electrochemiluminescence Detection in Aqueous-organic Media

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments