Research on the Role of Rat TPP-Ⅰ in Neuromedin B Degradation

Abstract

Abstract: TPP-Ⅰ(tripeptidyl peptidase-Ⅰ) protein turnover was studied by observing the role of rat TPP-Ⅰ in neuromedin B(NMB) and other neuropeptides degradations in some physiological situations. The mixtures of rat TPP-Ⅰ with each of NMB, other neuropeptides and Ala-Ala-Phe-MCA were made respectively under the same conditions. The reaction was observed at different timeand monitored by means of high performance liquid chromatography(HPLC) and time-of-flight mass spectrometry(TOF-MS) in vitro. NMB was broken down at the same degree as Ala-Ala-Phe-MCA by rat TPP-Ⅰ and Gly-Asn-Leu was released within 16 h, but other neuropeptides were not digested within 24 h. TPP-Ⅰ is the predominant proteolytic enzyme responsible for the intracellular degradation of neuromedin B. NMB has recently been found to be a good natural substrate for rat lysosomal TPP-Ⅰ.

Key words: Rat, Tripeptidyl peptidase-Ⅰ, Neuromedin B, Late-infantile neuronal ceroid lipofuscinosis, Degradation

DU Pei-ge, QIU Fang-ping, AN Li-ping, WANG Yan, LU Gang. Research on the Role of Rat TPP-Ⅰ in Neuromedin B Degradation[J]. Chemical Research in Chinese Universities, 2005, 21(3): 291-293.

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Research on the Role of Rat TPP-Ⅰ in Neuromedin B Degradation

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