Separation and Determination of Basesby Capillary Zone Electrophoresis

Chemical Research in Chinese Universities ›› 1997, Vol. 13 ›› Issue (3): 215-220.

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Separation and Determination of Basesby Capillary Zone Electrophoresis

ZHAO Tao, LIU Qi-ping, CHENG Jie-ke

Department of Chemistry, Wuhan University, Wuhan 430072

Received:1996-05-06 Online:1997-08-24 Published:2011-08-17
Supported by:
Supported by the National Natural Science Foundation of China.

Abstract

Abstract: The present paper covers the separation and determination of cytosine, 9-N-di- hydroxypropyltheophylline, theobromine, adenine, guanine, uracil, theophylline, 1-hydroxyethyl-5-fluorouracil, xanthine, uric acid, 6-mercaptopurine, 5-fluo- rouracil and 1 -propionyloxyuracil by capillary zone electrophoresis. In an uncoated silica capillary(70 cm ×75 μm i. d. effective length 63 cm), thirteen bases could be separated within 11 min under the optimum conditions : 0.01 mol/L(pH 10.0) bo-rate buffer, 40℃, 15 kV, 260 nm and 2 s load. Efficiencies up to 3.1 ×10⁵ theoreti-cal plates (4.9 ×10⁵ plates/m) were achieved.

Key words: Capillary zone electrophoresis, Base, Electrophoresis

ZHAO Tao, LIU Qi-ping, CHENG Jie-ke . Separation and Determination of Basesby Capillary Zone Electrophoresis[J]. Chemical Research in Chinese Universities, 1997, 13(3): 215-220.

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Separation and Determination of Basesby Capillary Zone Electrophoresis

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