Chemical Research in Chinese Universities ›› 2024, Vol. 40 ›› Issue (6): 1290-1297.doi: 10.1007/s40242-024-4140-3

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Construction of Photo- and Thermo-Responsive Polymer-MOF@Enzyme Composites for Enhancing Its Biocatalytic Performance

TAJWAR Muhammad Ali1,2, ALI Nasir1,2, ZHANG Xiangru1,2, JABEEN Rubina1,2, LIU Yutong1,3, SHANGGUAN Dihua1,2, QI Li1,2   

  1. 1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Bio-systems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China;
    2. School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, P. R. China;
    3. School of Pharmacy, Xinxiang Medical University, Xinxiang 453003, P. R. China
  • Received:2024-06-03 Online:2024-12-01 Published:2024-10-26
  • Contact: QI Li,qili@iccas.ac.cn E-mail:qili@iccas.ac.cn
  • Supported by:
    This work was supported by the National Natural Science Foundation of China (No. 22274159).

Abstract: Recently, metal-organic framework (MOF)@enzyme composites have attracted increasing research interest. However, the fabrication of polymers-modified MOF@enzyme composites with high bio-catalytic performance remains challenging. Herein, a stimulus-responsive polymer, poly(acrylate-3',3'-dimethyl-6-nitro-spiro-2H-1-benzopyran-2,2' indoline-1'-ester-co-N-isopropyl acrylamide) (PSPN), was in-situ polymerized in UiO-66-NH2 (UN). The dual-responsive PSPN-UN@L-ASNase composites were constructed following L-asparaginase (L-ASNase) covalently attached to the UN surface. Interestingly, under 365 nm UV irradiation at 45 ℃, the PSP moiety in PSPN underwent a trans-to-cis conformational change and the PN moiety in PSPN transferred from a stretched- to a coiled-state, generating a confinement effect that significantly enhanced the bio-catalytic performance of the composites. Compared with free L-ASNase, the composites showed a 42.0-folds increase in maximum catalytic reaction velocity. Furthermore, the PSPN-UN@L-ASNase composites demonstrated high toxicity for Jurkat leukaemia cells. The stimulus-responsive polymer-MOF@enzyme composites provide a novel avenue for controlled bio-catalysis with great potential for targeted leukaemia therapy.

Key words: Photo-/thermo-responsive polymer, Metal-organic framework (MOF), L-Asparaginase (L-ASNase) immobilization, Confinement effect, Catalytic efficiency, Leukaemia cell