Thermo-sensitive Porous Polymer Membrane-immobilized Cellulose as a Switchable Enzyme Reactor for Tuning Its Enzymolysis via Variation Temperature

doi:10.1007/s40242-023-3026-0

Chemical Research in Chinese Universities ›› 2023, Vol. 39 ›› Issue (6): 992-997.doi: 10.1007/s40242-023-3026-0

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Thermo-sensitive Porous Polymer Membrane-immobilized Cellulose as a Switchable Enzyme Reactor for Tuning Its Enzymolysis via Variation Temperature

QIAO Juan^1,2, ZHANG Xinya^1,3, CHENG Cheng^1,4, and QI Li^1,2

1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P.R. China;
2. School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, P.R. China;
3. School of Pharmacy, Xinxiang Medical University, Xinxiang 453003, P.R. China;
4. College of Chemistry & Environmental Science, Hebei University, Baoding 071002, P.R. China

Received:2023-02-07 Online:2023-12-01 Published:2023-11-18
Contact: QI Li E-mail:qili@iccas.ac.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (No.21727809).

Abstract

Abstract: Immobilization of enzymes onto porous membranes has attracted considerable attention in recent years. However, enhancing the enzymolysis efficiency of the resulting enzyme reactors by varying the environmental conditions poses a great challenge. In this work, poly(styrene-maleic anhydride-N,N-dimethylacrylamide) was prepared and utilized to construct a thermo-sensitive porous polymer membrane-based enzyme reactor (TS-PPMER) after cellulase was immobilized onto the support by covalent bonding. The catalytic activity of the nano-reactor was evaluated by measuring the yield of the product, glucose, at different temperatures with carboxymethylcellulose as the substrate. Interestingly, the polymer chains coiled and formed numerous nano-pores at a high temperature, which induced the confine effect and greatly boosted the enzymolysis efficiency of TS-PPMER. Furthermore, the proposed TS-PPMER was applied in the hydrolysis of green plant leaves in Epipremnum aureum. This work shows great potential in obtaining biological resources by an environmentally friendly approach using smart polymer-based nano-reactors.

Key words: Thermo-sensitive porous polymer membrane, Cellulase reactor, Catalytic efficiency, Glucose, Plant leaf

QIAO Juan, ZHANG Xinya, CHENG Cheng, and QI Li. Thermo-sensitive Porous Polymer Membrane-immobilized Cellulose as a Switchable Enzyme Reactor for Tuning Its Enzymolysis via Variation Temperature[J]. Chemical Research in Chinese Universities, 2023, 39(6): 992-997.

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Thermo-sensitive Porous Polymer Membrane-immobilized Cellulose as a Switchable Enzyme Reactor for Tuning Its Enzymolysis via Variation Temperature

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