Determination of Cysteine Using Near-infrared Diffuse Reflectance Spectroscopy with Enrichment via Thiol-maleimide Click Reaction

doi:10.1007/s40242-016-6279-z

Chemical Research in Chinese Universities ›› 2016, Vol. 32 ›› Issue (6): 912-916.doi: 10.1007/s40242-016-6279-z

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Determination of Cysteine Using Near-infrared Diffuse Reflectance Spectroscopy with Enrichment via Thiol-maleimide Click Reaction

WANG Cuicui¹, CAI Wensheng¹, SHAO Xueguang^1,2,3,4

1. Research Center for Analytical Sciences, College of Chemistry, Nankai University, Tianjin 300071, P. R. China;
2. Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 300071, P. R. China;
3. State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, P. R. China;
4. Collaborative Innovation Center of Chemical Science and Engineering, Nankai University, Tianjin 300071, P. R. China

Received:2016-06-30 Revised:2016-08-23 Online:2016-12-01 Published:2016-09-12
Contact: SHAO Xueguang, E-mail:xshao@nankai.edu.cn E-mail:xshao@nankai.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.21475068) and the Project of Innovation Team of the Ministry of Education of China(No.IRT13022).

Abstract

Abstract:

Enrichment technique has been proved to be an efficient way to make the near-infrared diffuse reflectance spectroscopy(NIRDRS) suitable for micro analysis. However, low selectivity presented by conventional enrichment methods makes the quantitative analysis easy to be affected by the coexisting components. In this study, a specific enrichment method with chemical bonding via thiol-maleimide click reaction was used to achieve the reduction of the interferences. Taking cysteine as the analyzing target, maleimide-functionalized SiO₂ nanoparticles were prepared for the enrichment of cysteine. Then determination of cysteine in aqueous solution and human serum was studied using the partial least squares model built from the NIRDRS spectra of the adsorbate. The results show that the concentration that can be quantitatively detected is as low as 2.0 μg/mL, and the correlation coefficient(R) between the reference and predicted concentration is 0.9871 for the validation samples. The recoveries are in the range of 89.5%-113.8% for human serum samples in the concentration range of 0-16.2 μg/mL.

Key words: Near-infrared diffuse reflectance spectroscopy, Click reaction, Cysteine, Multivariate calibration, Human serum

WANG Cuicui, CAI Wensheng, SHAO Xueguang. Determination of Cysteine Using Near-infrared Diffuse Reflectance Spectroscopy with Enrichment via Thiol-maleimide Click Reaction[J]. Chemical Research in Chinese Universities, 2016, 32(6): 912-916.

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Determination of Cysteine Using Near-infrared Diffuse Reflectance Spectroscopy with Enrichment via Thiol-maleimide Click Reaction

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