Spectrometric Study on the Interaction of Indocyanine Green with Human Serum Albumin

doi:10.1007/s40242-016-6008-7

Chemical Research in Chinese Universities ›› 2016, Vol. 32 ›› Issue (3): 343-347.doi: 10.1007/s40242-016-6008-7

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Spectrometric Study on the Interaction of Indocyanine Green with Human Serum Albumin

LI Xiaodong¹, FU Yu¹, MA Lina², WANG Zhenxin², ZHANG Huimao¹

1. Department of Radiology, the First Hospital of Jilin University, Changchun 130021, P. R. China;
2. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China

Received:2016-01-11 Revised:2016-03-24 Online:2016-06-01 Published:2016-04-05
Contact: ZHANG Huimao E-mail:huimaozhanglinda@163.com
Supported by:
Supported by the National Natural Science Foundation of China(No.81571737) and the Project of the Jilin Provincial Science and Technology Department, China(Nos.20130204028GX, 20140413037GH).

Abstract

Abstract:

The interaction of indocyanine green(ICG) with human serum albumin(HSA) was investigated via various spectrometric(UV-visible, fluorescence and circular dichroism) techniques. The experimental results indicate that the interaction of ICG with HSA depends on the values of R(R is defined as the molar ratio of HSA to ICG). The interaction of ICG with HSA can form two complexes with intrinsic binding constants(K_a) of 2.97×10⁵(R≤2) and 2.63×10⁴(R>2), respectively. The fluorescence and induced CD(ICD) spectra of ICG demonstrate that binding the first mole of HSA to ICG can form a chiral ICG-HSA complex with strong fluorescence emission, and the chirality and fluorescence of ICG-HSA complex can be significantly reduced by adding another mole of HSA to ICG. Furthermore, although both ICG and ICG-HSA complexes followed an energy-dependent endocytosis process to enter living cells, the cellular uptaken dynamic mechanism of ICG was significantly affected by the HSA conjugation.

Key words: Indocyanine green(ICG), Human serum albumin(HSA), Spectrometric study, Cellular uptaken

LI Xiaodong, FU Yu, MA Lina, WANG Zhenxin, ZHANG Huimao. Spectrometric Study on the Interaction of Indocyanine Green with Human Serum Albumin[J]. Chemical Research in Chinese Universities, 2016, 32(3): 343-347.

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Spectrometric Study on the Interaction of Indocyanine Green with Human Serum Albumin

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