In vitro Anticancer Efficacy by Magnetic Targeted Nanocarrier with Local Delivery of Paclitaxel

doi:10.1007/s40242-015-5115-1

Chemical Research in Chinese Universities ›› 2016, Vol. 32 ›› Issue (1): 149-154.doi: 10.1007/s40242-015-5115-1

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In vitro Anticancer Efficacy by Magnetic Targeted Nanocarrier with Local Delivery of Paclitaxel

ZHANG Shubin¹, QIAN Xue¹, ZHANG Daihui³, ZHU Jinming⁴, WU Yi², GUO Yi¹, XU Li¹

1. Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, P. R. China;
2. College of Pharmacy, Jilin University, Changchun 130021, P. R. China;
3. Jilin Entry-exit Inspection and Quarantine Bureau, Changchun 130062, P. R. China;
4. China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China

Received:2015-03-23 Revised:2015-09-15 Online:2016-02-01 Published:2015-12-28
Contact: WU Yi, GUO Yi E-mail:wuyi@jlu.edu.cn;guoyi@jlu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.81271697, 81571791, 31571017, 81171431), the Spe-cialized Research Fund for the Doctoral Program of Higher Education of China(Nos.20100061120077, 20120061110021), the Social Development Project of Science and Technology Department of Jilin Province, China(Nos.20120967, YYZX201264, 20130206069GX) and the “Significant New Drug Creation” Science and Technology Major Program of China (No.2012ZX09503001-003).

Abstract

Abstract:

A magnetic nanoparticles-loaded polymeric nanocarrier was developed. Amphiphilic copolymer, methoxy polyethylene glycol-poly(D,L-lactide-co-glycolide)(MPEG-PLGA) could self-assemble to form nanomicelle with the help of emulsion-solvent evaporation technique. This nanocarrier with core-shell structure was loaded with magnetic iron oxide nanoparticles(IONPs) and anticancer drug paclitaxel(PTX). The hydrodynamic diameter of IONPs-PTX-loaded nanocarrier showed an average size of 110 nm with a polydispersity index(PDI) of 0.136, and its zeta potential was (-4.76±0.36) mV. The drug-loading content and encapsulation efficiency were 4.47% and 31.28%, respectively. In vitro drug release experiment was performed and a sustained release profile was observed. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide(MTT) assay indicated that IONPs-PTX-loaded nanocarrier showed comparable cytotoxicity with free paclitaxel. When an external magnetic field was applied, the nanocarrier significantly localized at the target area, demonstrating that the nanocarrier could be used for potential magnetic targeted drug delivery.

Key words: Magnetic nanoparticle, Paclitaxel, Nanocarrier, Local delivery

ZHANG Shubin, QIAN Xue, ZHANG Daihui, ZHU Jinming, WU Yi, GUO Yi, XU Li. In vitro Anticancer Efficacy by Magnetic Targeted Nanocarrier with Local Delivery of Paclitaxel[J]. Chemical Research in Chinese Universities, 2016, 32(1): 149-154.

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In vitro Anticancer Efficacy by Magnetic Targeted Nanocarrier with Local Delivery of Paclitaxel

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