Interaction of Mixed Porphyrin-polypyridyl Ru(II) Complex with Bovine Serum Albumin

Chemical Research in Chinese Universities ›› 2010, Vol. 26 ›› Issue (5): 693-698.

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Interaction of Mixed Porphyrin-polypyridyl Ru(II) Complex with Bovine Serum Albumin

XU Lei1, LIU Ya-nan1, MEI Wen-jie1,2, HUANG Xiao-mei2, CHEN Tian-feng1, LIU Jie1* and ZHENG Wen-jie1*

1. Department of Chemistry, Jinan University, Guangzhou 510632, P. R. China;
2. School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, P. R. China

Received:2009-10-06 Revised:2009-12-09 Online:2010-09-25 Published:2010-11-26
Contact: LIU Jie. E-mail: tliuliu@jnu.edu.cn; ZHENG Wen-jie. E-mail: tzhwj@jnu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.20871056, 20771044 and 20901030), the Natural Science Foundation of Guangdong Province, China(Nos.8251063201000008 and 9451063201002077), the Planned Item of Science and Technology of Guangdong Province, China (No.2008A030201020) and the “211” Project Grant of Jinan University, China.

Abstract

Abstract: The interactions of mixed porphyrin-polypyridyl Ru(II) complexes [m(Py-3′)TPP-Ru(phen)2Cl]+(1) and its derivatives [Nim(Py-3′)TPP-Ru(phen)2Cl]+(2) and [Cum(Py-3′)TPP-Ru(phen)2Cl]+(3)(phen=1,10-phenanthroline; m(Py-3′)TPP=5-(3′-pyridyl)-10,15,20-triphenylporphyrin) with bovine serum albumin(BSA) were investigated by fluorescence, UV-Vis and circular dichroism(CD) spectroscopies. The UV-Vis and CD spectral experiments indicated that the secondary structures of the protein were perturbed in the presence of the porphyrin Ru(II) complex and the perturbation was enhanced under the irradiation with ultra-violet light. The fluorescence quenching mechanism of BSA by the three complexes was determined to be a static process, and the apparent binding constant K values for complexes 1, 2 and 3 measured by fluorescence quenching method were (3.86±0.03)×103 L/mol(n=0.94±0.04), (5.69±0.04)×103 L/mol(n=1.03±0.06), and (6.54±0.02)×103 L/mol(n=1.03±0.05), respectively.

Key words: Porphyrin , Bovine serum albumin(BSA), Ru(II) complex, Binding

XU Lei1, LIU Ya-nan1, MEI Wen-jie1,2, HUANG Xiao-mei2, CHEN Tian-feng1, LIU Jie1* and ZHENG Wen-jie1*. Interaction of Mixed Porphyrin-polypyridyl Ru(II) Complex with Bovine Serum Albumin[J]. Chemical Research in Chinese Universities, 2010, 26(5): 693-698.

References

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Interaction of Mixed Porphyrin-polypyridyl Ru(II) Complex with Bovine Serum Albumin

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