Adsorption/Desorption of Low-density Lipoprotein on a Heparinized Surface of Gold Sensors

Chemical Research in Chinese Universities ›› 2012, Vol. 28 ›› Issue (2): 323-328 .

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Adsorption/Desorption of Low-density Lipoprotein on a Heparinized Surface of Gold Sensors

LAN Ping^1,2, JI Jing², HUANG Xiao-Jun², GUDURU Deepak³, GROTH Thomas³, VIENKEN Jörg⁴, DING Hui⁵

1. College of Materials and Textiles, Jiaxing University, Jiaxing 314001, P. R. China;
2. Key Laboratory of Macromolecular Synthesis and Functionalization, Ministry of Education, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, P. R. China;
3. Biomedical Materials Group, Department of Pharmaceutics and Biopharmaceutics, Institute of Pharmacy, Martin Luther University Halle-Wittenberg, Halle(Saale) 06120, Germany;
4. Fresenius Medical Care of Deutschland GmbH, BioSciences Department, Bad Homburg 61352, Germany;
5. College of Mathematics Physics and Information Engineering, Jiaxing University, Jiaxing 314001, P. R. China

Received:2011-08-15 Revised:2011-11-20 Online:2012-03-25 Published:2012-03-08
Supported by:
Supported by the Natural Science Foundation of Zhejiang Province of China(Nos.Y4110606 and Y1090649), the Opening Foundation of Zhejing Provincial Top Key Discipline, China(No.20110915), the Zhejiang Provincial Innovative Research Team, China(No.2009R50004) and the Fresenius Medical Care of Deutschland GmbH's Nephrocore Program, Germany.

Abstract

Abstract: Heparin has been considered to be a potentially useful ligand for low-density lipoprotein(LDL) detection and analysis in a clinical context. In order to construct an affinity surface for preferential adsorption of LDL, heparin-modified gold surface(GS-Hep) was fabricated by a self-assembling method and hydrophobic-modified gold surfaces(GS-Hydro) was used as a control. The morphologies of the modified gold surfaces were investigated by atomic force microscopy(AFM) and the quantity of heparin bound to gold surface was assayed by the toluidine blue(TB) colorimetric method. Water contact angles were determined to investigate wettability on GS-Hep and GS-Hydro. Surface plasmon resonance(SPR) technique was used subsequently to detect the selective binding of LDL with heparin. And the investigation on the effect of pH on LDL adsorption suggests that lower pH lead to higher quantities of LDL adsorption on GS-Hep. Compared with GS-Hydro, GS-Hep is selective for LDL from both single and binary protein solutions. Moreover, adsorbed LDL on GS-Hep could be washed off by injecting elution solution, such as NaCl solution, for the purpose of the regeneration of GS-Hep for further LDL adsorption.

Key words: Adsorption/desorption, Low-density lipoprotein(LDL), Heparinized surface, Surface plasmon resonance

LAN Ping, JI Jing, HUANG Xiao-Jun, GUDURU Deepak, GROTH Thomas, VIENKEN Jörg, DING Hui . Adsorption/Desorption of Low-density Lipoprotein on a Heparinized Surface of Gold Sensors[J]. Chemical Research in Chinese Universities, 2012, 28(2): 323-328 .

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Adsorption/Desorption of Low-density Lipoprotein on a Heparinized Surface of Gold Sensors

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