Label-free Detection of Biotin Using Nanoporous TiO2/DNA Thin-film Coated Wavelength Interrogated Surface Plasmon Resonance Sensors

doi:10.1007/s40242-014-3312-y

Chemical Research in Chinese Universities ›› 2014, Vol. 30 ›› Issue (1): 157-162.doi: 10.1007/s40242-014-3312-y

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Label-free Detection of Biotin Using Nanoporous TiO₂/DNA Thin-film Coated Wavelength Interrogated Surface Plasmon Resonance Sensors

LI Qiushun¹, HE Hao², WANG Junnan³, ZHENG Hui¹, SHI Jianguo¹, LI Mingyu⁴, DONG Wenfei², QI Zhimei⁵

1. Key Laboratory of Biosensor of Shandong Province, Biology Institute of Shandong Academy of Sciences, Jinan 250014, P. R. China;
2. Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, P. R. China;
3. The Second Hospital of Jilin University, Changchun 130041, P. R. China;
4. State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou 310027, P. R. China;
5. State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, P. R. China

Received:2013-07-22 Revised:2013-10-02 Online:2014-02-01 Published:2013-11-25
Contact: WANG Junnan,E-mail:junnanwang1973@gmail.com E-mail:junnanwang1973@gmail.com
Supported by:
Supported by the National Natural Science Foundation of China(Nos.91123029, 61077066, 61340032), the National High Technology Research and Development Program of China(No.2012AA063302) and the Natural Science Foundation of Shandong Province, China(No.ZR2012CM029).

Abstract

Abstract:

The sensing sensitivity of the wavelength interrogated surface plasmon resonance(WISPR) biosensor could be improved by self-assembly of nanoporous thin-film of TiO₂ nanoparticles/DNA(TiO₂/DNA)_n(n is the number of bilayer) on wavelength interrogated surface plasmon resonance(WISPR) chips. The growth behavior and surface structure of the nanoporous thin-film were investigated by UV-Vis spectroscopy and scanning electron microscopy, respectively. The home-made WISPR sensor with Krestchmann configuration consisted of a tungsten-halogen lamp as a photon source and a CCD camera as the detector. After the deposition of (DNA/TiO₂)_n thin film on WISPR chips, the resonance peak of the reflection spectra appeared in air. With the increases of n, the resonance wavelength gradually red shifted, which is consistent with the simulated results. After the optimization of the porous film, the WISPR biosensor was utilized to detect low-molecular-weight analytes, such as biotin. The result demonstrates that the sensitivity of [poly(styrene sulfonate)/polyally lamine hydrochlorides]₅(PSS/PAH)₅ could be 4 times higher than that of polyelectrolyte multilayer modified WISPR sensor.

Key words: Nanoporous film, Sensing sensitivity, Biotin, Wavelength interrogated surface plasmon resonance

LI Qiushun, HE Hao, WANG Junnan, ZHENG Hui, SHI Jianguo, LI Mingyu, DONG Wenfei, QI Zhimei. Label-free Detection of Biotin Using Nanoporous TiO₂/DNA Thin-film Coated Wavelength Interrogated Surface Plasmon Resonance Sensors[J]. Chemical Research in Chinese Universities, 2014, 30(1): 157-162.

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Label-free Detection of Biotin Using Nanoporous TiO₂/DNA Thin-film Coated Wavelength Interrogated Surface Plasmon Resonance Sensors

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