Tumor Cell Detection Device Based on Surface Plasmon Resonance Imaging and Image Processing

doi:10.1007/s40242-014-3415-5

Chemical Research in Chinese Universities ›› 2014, Vol. 30 ›› Issue (2): 211-215.doi: 10.1007/s40242-014-3415-5

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Tumor Cell Detection Device Based on Surface Plasmon Resonance Imaging and Image Processing

YANG Mengchao¹, JIN Xinyu², YUAN Maokai¹, LIU Fan², LANG Yicong², WU Zhongyu¹, XIANG Liancheng¹, ZHOU Chao¹, OUYANG Bo², JIN Wei¹, MU Ying¹

1. Research Center for Analytical Instrumentation, Institute of Cyber-systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, P. R. China;
2. Department of Information Science & Electronic Engineering, Zhejiang University, Hangzhou 310027, P. R. China

Received:2013-09-29 Revised:2013-12-07 Online:2014-04-01 Published:2013-12-11
Contact: JIN Xinyu, MU Ying E-mail:muying@zju.edu.cn;jinxy@zju.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.31070772, 31270907, 21275129).

Abstract

Abstract:

A laboratory-made tumor cell detection device was fabricated based on both surface plasmon resonance imaging(SPRi) and image processing. In this device, a gravity-induced flow injection chip(gFIC) was exploited to replace a pump. Also two charge coupled devices(CCDs) were used to detect HepG2 cells by SPRi and image processing, respectively. The results of two CCDs are associated. Protein A was used to modify the sensing surface. The inlet angle was carefully adjusted for the device to get an enhanced image. In the test, the contrast among cell solutions at different concentrations can be easily distinguished. The other CCD using image processing can tell false-positive in some degree. This detection is label-free, real time, and precise.

Key words: Surface plasmon resonance, Image processing, Tumor detection, Gravity induced

YANG Mengchao, JIN Xinyu, YUAN Maokai, LIU Fan, LANG Yicong, WU Zhongyu, XIANG Liancheng, ZHOU Chao, OUYANG Bo, JIN Wei, MU Ying. Tumor Cell Detection Device Based on Surface Plasmon Resonance Imaging and Image Processing[J]. Chemical Research in Chinese Universities, 2014, 30(2): 211-215.

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Tumor Cell Detection Device Based on Surface Plasmon Resonance Imaging and Image Processing

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