Preparation and Electrochemical Behaviors of AA-PEGMA Copolymer Film Electrode

Abstract

Abstract: A new kind of comblike copolymer film composed of acrylic acid-polyethylene glycol monomethyl ether acrylate copolymer(AA-PEGMA copolymer) was successfully synthesized to immobilize hemoglobin(Hb). FTIR, UV-Vis and CD spectra suggest that Hb kept its original structure in the AA-PEGMA copolymer film without denaturation. A pair of well-defined, quasi-reversible cyclic voltammetric peaks at around –270 mV vs. saturated calomel electrode(SCE) for the Hb Fe(Ⅲ)/Fe(Ⅱ) redox couple was observed on the film-modified electrode in phosphate buffer solution(PBS, pH=7.0). The formal potential of Hb/AA-PEGMA copolymer film-modified electrode is linearly dependent on solution pH with a slope of –46.3 mV/pH, illustrating that one-proton transfer was accompanied with each electron transfer. Furthermore, the modified electrode displayed electrocatalytic response to the reduction of H₂O₂ with a linear range of 3.5―126 μmol/L and a detection limit of 1.17 μmol/L. In conclusion, the AA-PEGMA copolymer film was proved to be an excellent matrix for the immobilization and electrochemistry of proteins.

Key words: Copolymer film, Hemoglobin, Comblike polymer, Electrochemical behavior

L� Xue, LIU Feng-qi. Preparation and Electrochemical Behaviors of AA-PEGMA Copolymer Film Electrode[J]. Chemical Research in Chinese Universities, 2012, 28(1): 156-161.

References

[1] Wang S. F., Chen T., Zhang Z. L., Shen X. C., Lu Z. X., Pang D. W.,Wong K. Y., Langmuir, 2005, 21(20), 9260 [2] Yao H., Li N., Xu J. Z., Zhu J. J., Talanta, 2007, 71(2), 550 [3] Wang Q. L., Lu G. X., Yang B. J., Biosens. Bioelectron., 2004,19(10), 1269 [4] Zeng X., Wei W., Li X., Zeng J. X., Wu L., Bioelectrochemistry,2007, 71(2), 135 [5] Kafi A. K. M., Lee D. Y., Park S. H., Kwon Y. S., Thin Solid Films,2007, 515(12), 5179 [6] Miscoria S. A., Barrera G. A., Sens. Actuators, B, Chem., 2006,115(1), 205 [7] Sun W., Wang D. D., Gao R. F., Jiao K., Electrochem. Commun.,2007, 9(5), 1159 [8] Zhang L., Zhang Q., Li J. H., Adv. Funct. Mater., 2007, 17(12), 1958 [9] Huang H., Hu N. F., Zeng Y. H., Zhou G., Anal. Biochem., 2002,308(1), 141 [10] Guo S. J., Dong S. J., Trends Anal. Chem., 2009, 28(1), 96 [11] Shan D., Wang S. X., Xue H. G., Gosnier S., Electrochem. Commun.,2007, 9(4), 529 [12] Chattopadhyay K., Mazumdar S., Bioelectrochemistry, 2005, 53(1),2001 [13] Li G. P., Du L. W., Chen H. J., Zhang L. X., Wang E. K., Electroanalysis,2008, 20(20), 2171 [14] Sun W., Gao R. F., Jiao K., Electroanalysis, 2007, 19(13), 1368 [15] Kong Y. T., Boopathi M. B., Shim Y., Biosens. Bioelectron., 2003,19(3), 227 [16] Shan D., Wang S. X., Zhu D. B., Xue H. G., Bioelectrochemistry,2007, 71(2), 198 [17] Chen L., Lu G., J. Electroanal. Chem., 2006, 597(1), 51 [18] Zhang Q., Lv X., Qiao Y., Zhang L., Liu D. L., Zhang W., Han G. X.,Song X. M., Electroanalysis, 2010, 22(9), 1000 [19] Kizhakkedathu J. N., Janzen J., Le Y., Kainthan R. K., Brooks D. E.,Langmuir, 2009, 25(6), 3794 [20] Xu Y., Peng W. L., Liu X. J., Li G. X., Biosens. Bioelectron., 2004,20(3), 533 [21] Jiang G. Q., Liu C., Liu X. L., Chen Q. R., Zhang G. H., Yang M.,Liu F. Q., Polymer, 2010, 51(6), 1507 [22] Kong X. M., Hu B., Cao E. X., Hao X. Y., Xie X. M., Acta Polym.Sinica, 2010, 2, 160 [23] Stephen W. P., Jurgen G., Biochemistry, 1981, 20(1), 33 [24] Zhang Q., Zhang L., Li J. H., Electrochim. Acta, 2008, 53(7), 3050 [25] Greve J., Maestre M. F., Moise H., Hosoda J., Biochemistry, 1978,17(5), 887 [26] Zhou J., Jia G. Q., Feng Z. C., Li C., Chem. J. Chinese Universities,2010, 31(2), 309 [27] Jiang J. G., Wang Z. X., Liu C. W., Liu D. J., Yang X. R., Dong S. J.,Chem. J. Chinese Universities, 2001, 22(7), 1131 [28] Chattopadhyay K., Mazumdar S., Biochemistry, 2000, 39(1), 263 [29] Whitmore L., Wallace B. A., Nucleic Acids Res., 2004, 32, W668 [30] Chen Y. Y., Huang L., Ji X. T., Lin Q., Chen P., Ke C. H., Huang H.Q., Chem. J. Chinese Universities, 2010, 31(5), 896 [31] Guo W., Wu Y. Q., Zheng L. Y., Xu L. R., Fan X. L., Chem. J. ChineseUniversities, 2009, 30(7), 1314 [32] Shan D., Cheng G. X., Zhu D. B., Xue H. G., Cosnier S., Ding S. N.,Sens. Actuators, B, Chem., 2009, 137(1), 259 [33] Nassar A. E. F., Zhang Z., Hu N. F., Rusling J. F., J. Phys. Chem. B,1997, 101(12), 2224 [34] Xu Y. X., Hu C. G., Hu S. S., Bioelectrochemistry, 2009, 74(2), 254 [35] Shen L., Hu N. F., Biochim. Biophys. Acta, 2004, 1608(1), 23 [36] Kumar S. A., Chen S. M., Talanta, 2007, 72(2), 831 [37] Zhao Y. D., Bi Y. H., Zhang W. D., Luo Q. M., Talanta, 2005, 65(2),489 [38] Gu H. Y., Yu A. M., Chen H. Y., J. Electroanal. Chem., 2001, 516(1),119 [39] Jia N. Q., Lian Q., Wang Z. Y., Shen H. B., Sens. Actuators, B, Chem.,2009, 137(1), 230 [40] Yang X. S., Chen X., Yang L., Yang W. S., Bioelectrochemistry, 2008,74(1), 90 [41] Wen Y. L., Wu H. X., Chen S. H., Lu Y. H., Shen H. B., Jia N. Q.,Electrochim. Acta, 2009, 54(27), 7078