CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (5): 836-840.

• Articles • Previous Articles     Next Articles

Induction of Effective Antitumor Immune Response by Combined Administration of hIL-18 and NDV HN

HUANG Hai-yan1,2,5, MENG Xiang-wei1, LI Xiao1,2,3, SUN Li-li2,4, KAN Shi-fu2,3, LIU Lei1,2, PIAO Bing-guo1,2, YANG Guo-hua1,2, WANG Zhuo-yue2,3, WANG Yu-hang2,3, QI Yan-xin2,3 and JIN Ning-yi2,3*   

  1. 1. Department of Gastroenterology, the First Hospital of Jilin University, Changchun 130021, P. R. China;
    2. Laboratory of Genetic Engineering of PLA,
    3. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Institute, Academy of Military Medical Sciences, Changchun 130062, P. R. China;
    4. Department of Head and Neck Surgery, Tumor Hospital of Jilin Province, Changchun 130012, P. R. China;
    5. Digestive Internal Medicine, Xuzhou Center Hospital, Xuzhou 221009, P. R. China
  • Received:2011-01-18 Revised:2011-03-30 Online:2011-09-25 Published:2011-09-06
  • Contact: JIN Ning-yi E-mail:ningyik@126.com
  • Supported by:

    Supported by the Genetically Modified Organisms Breeding Major Projects, China(No.2009ZX08006-002B), the Key Technologies Research and Development Program of Jilin Province, China(No.10ZDGG007) and the Postdoctoral Science Foundation Funded Project of China(No.20100481057).

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Abstract: To analyze the antitumor potential and mechanism of action of simultaneous Newcastle disease virus (NDV) hemagglutinin-neuraminidase(HN) and human interleukin 18(hIL-18) gene transfer in C57BL/6 mice with H22 hepatoma，the mouse model with H22 hepatoma was established in C57BL/6 mice, and the antitumor effects of the combined application of NDV HN and hIL-18 were evaluated in vivo. The results show that the growth of established tumors in mice immunized with adenovirus(Ad)-HN in conjunction with Ad-hIL-18 was significantly inhibited compared with that in mice immunized with Ad-HN, Ad-hIL-18 alone, or the empty vector(Ad-mock). Furthermore, the immunization of mice with Ad-HN in conjunction with Ad-hIL-18 elicited strong natural killer activity and H22 tumor-specific cytotoxic T lymphocyte(CTL) responses in vivo. In addition, T cells from the lymph nodes of mice immunized with Ad-hIL-18 or Ad-HN+Ad-hIL-18 secreted high levels of the Th1 cytokine IL-2 and interferon-γ (IFN-γ), indicating that the regression of tumor cells is related to a Th1-type dominant immune response. These results demonstrate that vaccination with NDV HN together with hIL-18 may be a novel and powerful strategy for cancer immunotherapy.

Key words: Newcastle disease virus, Human interleukin 18(hIL-18), Hemagglutinin-neuraminidase(HN), Hepatoma, Antitumor immunity