Antitumor Effects of Newcastle Disease Virus Hemagglutinin-neuraminidase Used as a Molecular Adjuvant

doi:10.1007/s40242-013-2391-5

Chemical Research in Chinese Universities ›› 2013, Vol. 29 ›› Issue (2): 270-274.doi: 10.1007/s40242-013-2391-5

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Antitumor Effects of Newcastle Disease Virus Hemagglutinin-neuraminidase Used as a Molecular Adjuvant

JI Hui-fan^1,2, CHI Bao-rong¹, HE Dong-yun^1,2, LI Chang^2,3, HU Ning-ning², WANG Kai², SHENG Yuan², WANG Hong-yu², JIN Ning-yi^2,3

1. Department of Gastroenterology, the First Hospital of Jilin University, Changchun 130021, P. R. China;
2. Institute of Military Veterinary, Academy of Military Medical Sciences of PLA, Changchun 130122, P. R. China;
3. Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 130021, P. R. China

Received:2012-10-15 Revised:2012-12-16 Online:2013-04-01 Published:2013-03-20
Supported by:
Supported by the National Science and Technology Major Projects for Major New Drugs Innovation and Development of China(No.2010ZX09401-305-14), the National Natural Science Foundation of China(Nos.81072210, 81101140) and the Key Technologies R&D Program of Jilin Province of China(Nos.10ZDGG007, 201015166, 201101066).

Abstract

Abstract:

Gene therapy is a potentially powerful tool used in cancer therapy. The strength of immune responses induced by some strategies is usually low, therefore, the development of agents capable of enhancing these responses is highlighted. The authors investigated the potential of an approach based on the hemagglutinin-neuraminidase(HN) of Newcastle disease virus(NDV) as a potential immune adjuvant. It was found that recombinant adenovirus(Ad) infected SGC7901 cells expressing HN exhibited both hemagglutinin(HA) and neuraminidase(NA) activities. It was demonstrated that administration of HN induced higher levels of the effector cytokines TNF-α, IFN-α and IFN-γ and increased natural killer(NK) cell activity. Based on the therapeutic tumor model, the results show that the administration of HN with Apoptin led to improved survival and tumor suppression. In conclusion, this study indicates that HN stimulates innate immune responses to make the activity of NK cells increased, which highlights the potential adjuvant activity of HN in cancer gene therapy.

Key words: Hemagglutinin-neuraminidase(HN), Adjuvant, Innate immunity, Natural killer cell, Antitumor immunity

JI Hui-fan, CHI Bao-rong, HE Dong-yun, LI Chang, HU Ning-ning, WANG Kai, SHENG Yuan, WANG Hong-yu, JIN Ning-yi. Antitumor Effects of Newcastle Disease Virus Hemagglutinin-neuraminidase Used as a Molecular Adjuvant[J]. Chemical Research in Chinese Universities, 2013, 29(2): 270-274.

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Antitumor Effects of Newcastle Disease Virus Hemagglutinin-neuraminidase Used as a Molecular Adjuvant

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