Toxicity of Naphthalene on Caenorhabditis elegans

Abstract

Abstract: Naphthalene is a common environmental contaminant substance. The toxic effects of naphthalene on Caenorhabditis elegans were investigated at the molecular, biochemical and physiological levels. To assess the molecular-level effect, stress-related gene expression was investigated such as those of hsp-16.1, sod-3, ctl-2, cep-1, cyp35a2, ugt-44, gst-1 and dhs-28. Cell apoptosis was assessed at the biochemical level. Life span, locomotion behaviors and brood size were investigated at the physiological level. The results indicate that naphthalene exposure could not only induce the expression of stress-related genes such as hsp16.1, sod-3, ctl-2 and cep-1 but also reduce the life span of Caenorhabditis elegans. At the same time, naphthalene exposure could result in cell apoptosis and interfere in the locomotion behaviors of Caenorhabditis elegans. These data suggest that naphthalene has multiple toxicity on Caenorhabditis elegans.

Key words: Naphthalene, Caenorhabditis elegans, Toxicity, Real-time polymerase chain reaction(RT-PCR)

ZHANG Shu-hua, XU Jing-bo*, LIU Cheng-bai, LI Qiao, GUAN Shu-wen, .... Toxicity of Naphthalene on Caenorhabditis elegans[J]. Chemical Research in Chinese Universities, 2011, 27(3): 468-473.

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