CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (3): 455-460.

• Articles • Previous Articles     Next Articles

Activity of T Cells Stimulated by Hemagglutinin-neuraminidase of Newcastle Disease Virus in vivo

PIAO Bing-guo1,2,5, LI Xiao2,3, SUN Li-li2,4, KAN Shi-fu2,3, LIU Lei1,2, HUANG Hai-yan1,2, YANG Guo-hua1,2, WANG Yu-hang2,3, WANG Zhuo-yue2,3, SUN Jiu-hua2,5, PIAO Yun-feng1,2 and JIN Ning-yi2,3*   

  1. 1. The First Hospital of Jilin University, Changchun 130021, P. R. China;
    2. Genetic Engineering Laboratory of PLA,
    3. Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Military Veterinary Institute, Academy of Military Medical Sciences of PLA, Changchun 130062, P. R. China;
    4. Head and Neck Surgery, Tumor Hospital of Jilin Province, Changchun 130001, P. R. China;
    5. Department of Gastroenterology, 209 Hospital of PLA, Mudanjiang 157000, P. R. China
  • Received:2010-12-08 Revised:2011-03-15 Online:2011-05-25 Published:2011-04-29
  • Contact: JIN Ning-yi E-mail:ningyik@126.com
  • Supported by:

    Supported by the Genetically Modified Organisms Breeding Major Project of China(No.2009ZX08006-002B) and the Key Technologies Research and Development Programme of Jilin Province, China(No.10ZDGG007).

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Abstract: To investigate the stimulated activity of T cells and the anti-tumor properties of hemagglutinin-neurami- nidase(HN) of Newcastle disease virus(NDV) strain Changchun(NDVcc), the expression of HN gene in hepatoma cells(human HepG-2 and mouse H22 cells) infected with the recombinant adenovirus(Ad-HN) was identified by Western blot analysis and flow cytometry. Sialidase activity of NDVcc HN expressed by Ad-HN was assayed by the periodate-resorcinol method. The in vivo anti-tumor effects of NDVcc HN were evaluated in the H22 solid tumor model. Regional lymph nodes of the mouse model treated with Ad-HN were removed to harvest T lymphocytes and evaluating the specific cytotoxicity of cytotoxic T lymphocyte(CTL) and natural killer(NK) cells by an L-lactate dehydrogenase(LDH) assay, in the mean time, the secretion of cytokines was analyzed by enzyme linked immunosorbent assays(ELISA). The results show that NDVcc HN was effectively expressed by Ad-HN in HepG-2 and H22 cells. The sialidase activity assay showed that Ad-HN significantly reduced sialic acid level of the hepatoma cells compared with the cells infected the empty adenovirus vector(Ad-mock). When treated with Ad-HN, the growth of subcutaneous H22 primary tumors in C57BL/6 mice was suppressed, and the mean mice survival increased. In addition, the treatment of Ad-HN elicited strong NK and CTL responses, and high levels of Th1 cytokines, such as IL-2 and IFN-γ. In conclusion, NDVcc HN effectively elicits T cell-mediate anti-tumor cytotoxicity via sialidase activity and may be a novel strategy for cancer immunotherapy.

Key words: Newcastle disease virus, Hemagglutinin-neuraminidase, Hepatoma, T Cell, Anti-tumor immunity