Revealing the Cell Entry Dynamic Mechanism of Single Rabies Virus Particle

doi:10.1007/s40242-022-2069-y

Chemical Research in Chinese Universities ›› 2022, Vol. 38 ›› Issue (3): 838-842.doi: 10.1007/s40242-022-2069-y

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Revealing the Cell Entry Dynamic Mechanism of Single Rabies Virus Particle

LI Siying¹, PAN Yangang², TENG Honggang², SHAN Yuping¹, YANG Guocheng¹, and WANG Hongda²

These authors contributed equally to this work. 1. School of Chemistry and Life Science, Advanced Institute of Materials Science, Changchun University of Technology, Changchun 130012, P. R. China;
2. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China

Received:2022-03-02 Revised:2022-03-31 Online:2022-06-01 Published:2022-05-26
Contact: WANG Hongda, SHAN Yuping E-mail:hdwang@ciac.ac.cn;shanyp@ciac.ac.cn
Supported by:
This work was supported by the Project of the Education Department of Jilin Province, China(No.JJKH20220666KJ), the Talent of Jilin Province Development Fund Project, China(No.2021Y004), and the National Natural Science Foundation of China(No.21773017).

Abstract

Abstract: The rabies virus is a neurotropic virus that causes fatal diseases in humans and animals. Although studying the interactions between a single rabies virus and the cell membrane is necessary for understanding the pathogenesis, the internalization dynamic mechanism of single rabies virus in living cells remains largely elusive. Here, we utilized a novel force tracing technique based on atomic force microscopy(AFM) to record the process of single viral entry into host cell. We revealed that the force of the rabies virus internalization distributed at (65±25) pN, and the time was identified by two peaks with spacings of (237.2±59.1) and (790.3±134.4) ms with the corresponding speed of 0.12 and 0.04 μm/s, respectively. Our results provide insight into the effects of viral shape during the endocytosis process. This report will be meaningful for understanding the dynamic mechanism of rabies virus early infection.

Key words: Rabies virus, Entering host cell, Dynamic mechanism, Atomic force microscopy(AFM), Force tracing

LI Siying, PAN Yangang, TENG Honggang, SHAN Yuping, YANG Guocheng, and WANG Hongda. Revealing the Cell Entry Dynamic Mechanism of Single Rabies Virus Particle[J]. Chemical Research in Chinese Universities, 2022, 38(3): 838-842.

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Revealing the Cell Entry Dynamic Mechanism of Single Rabies Virus Particle

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