Lentivirus-mediated RNA Interference and Over-expression of CDK2AP1 CDNA Regulate CDK2AP1 Expression in Human Lung Cancer A549 Cells

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (3): 445-449.

• Articles • Previous Articles Next Articles

Lentivirus-mediated RNA Interference and Over-expression of CDK2AP1 CDNA Regulate CDK2AP1 Expression in Human Lung Cancer A549 Cells

GAO Nan¹, ZHANG Xing-yi¹, JIANG Rui², WANG Guan³, LI Jin-dong¹, JIN Cheng-yan¹ and SUN Mei^4*

1. Department of Thoracic Surgery, the Second Hospital of Jilin University, Changchun 130041, R. P. China;
2. Department of Orthopedic Surgery, China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
3. Department of Cardiac Medicine, 4. Department of Pathology, the Second Hospital of Jilin University, Changchun 130041, P. R. China

Received:2011-01-18 Revised:2011-03-25 Online:2011-05-25 Published:2011-04-29
Contact: SUN Mei E-mail:sun.mei55@yahoo.com
Supported by:
Supported by the Grants from the Project of Scientific Innovation and Creative for Jilin Provincial Oversea Scholars(No.2010273), Jilin Provincial Science and Technology Services(Nos.20050408, 20070720, 200805120, 20090732), Jilin Provincial Development and Reformation Committee(No.2009Y042J12314), Jilin Province Talent Development Foundation (No.JRJB2007-2) and the Natural Science Foundation of China(Nos.30670301, 30870354).

Abstract

Abstract: Cyclin-dependent kinase 2-associated protein 1(CDK2AP1), a cell growth inhibitory factor, is abnormally expressed in cancer cells, and might be implicated in the development of lung cancer. However, no studies on the function of CDK2AP1 in human lung cancer have been yet reported. In this study, overexpressing lentiviral vectors containing full-length CDK2AP1 cDNA and CDK2AP1 shRNA(short hairpin RNA) were constructed. Our results show that infecting A549 cells with lentivirus containing CDK2AP1 shRNA or full-length CDK2AP1 cDNA results in significantly down- or up-regulated the expression of CDK2AP1, respectively, thereby providing reliable tools for studying the function of CDK2AP1 in pulmonary carcinogenesis. Our data of MTT assay and flowcytometric analysis demonstrate that CDK2AP1 plays an important role in the proliferation/growth and cell cycling of A549 cells in vitro, and further investigation into its underlying mechanism of pulmonary carcinogenesis is needed.

Key words: Lung, Cancer, CDK2AP1, shRNA, Lentivirus

GAO Nan, ZHANG Xing-yi, JIANG Rui, WANG Guan, LI Jin-dong, JIN Cheng-yan and SUN Mei*. Lentivirus-mediated RNA Interference and Over-expression of CDK2AP1 CDNA Regulate CDK2AP1 Expression in Human Lung Cancer A549 Cells[J]. Chemical Research in Chinese Universities, 2011, 27(3): 445-449.

[1]	XIAO Chulei, CHI Quan, WANG Xian. Recent Progress in Mass Spectrometry-based Metabolomics for Colorectal Cancer [J]. Chemical Research in Chinese Universities, 2022, 38(4): 886-893.
[2]	HUANG Qin, LIU Xin, ZHANG Pengge, WU Zhan, ZHAO Zilong. A DNA Nano-train Carrying a Predefined Drug Combination for Cancer Therapy [J]. Chemical Research in Chinese Universities, 2022, 38(4): 928-934.
[3]	CHEN Minwen, LIAO Tao, ZENG Linsheng, ZENG Zhongyi, YANG Qinglai, WANG Guoxin. Plasmonic Gold Chip for Multiplexed Detection of Ovarian Cancer Biomarker in Urine [J]. Chemical Research in Chinese Universities, 2022, 38(4): 935-940.
[4]	LIU Qianyu, DU Zhenwu, ZHU He, ZHAO Tianyu, PAN Ying. Comprehensive Analysis of Differential Gene Expression Profile via RNA Sequencing in the Human Ovarian Cancer SKOV3 Cells Treated with Simvastatin [J]. Chemical Research in Chinese Universities, 2022, 38(2): 632-638.
[5]	LI Duo, WU Chao, TANG Xuehui, ZHANG Yue, WANG Tie. Electrochemical Sensors Applied for In vitro Diagnosis [J]. Chemical Research in Chinese Universities, 2021, 37(4): 803-822.
[6]	CHU Binbin, WANG Houyu, HE Yao. Fluorescent Silicon-based Nanomaterials Imaging Technology in Diseases [J]. Chemical Research in Chinese Universities, 2021, 37(4): 880-888.
[7]	LI Mengqi, MA He, SHI Chao, ZHANG Han, LONG Saran, SUN Wen, DU Jianjun, FAN Jiangli, PENG Xiaojun. A Cyanine-based Liposomal Nanophotosensitizer for Enhanced Cancer Chemo-Photodynamic Therapy [J]. Chemical Research in Chinese Universities, 2021, 37(4): 925-933.
[8]	CUI Min, REN Jujie, WEN Xiaofang, LI Na, XING Yifei, ZHANG Cong, HAN Yuanyuan, JI Xueping. Electrochemical Detection of Superoxide Anion Released by Living Cells by Manganese(III) Tetraphenyl Porphine as Superoxide Dismutase Mimic [J]. Chemical Research in Chinese Universities, 2020, 36(5): 774-780.
[9]	LIU Jie, CHEN Bo, ZHANG Jianjun. Preparation of pH-Responsive Doxorubicin Nanocapsules by Combining High-gravity Antisolvent Precipitation with In-situ Polymerization for Intracellular Anticancer Drug Delivery [J]. Chemical Research in Chinese Universities, 2020, 36(5): 927-933.
[10]	FU Xuancheng, BAI Haotian, LYU Fengting, LIU Libing, WANG Shu. Conjugated Polymer Nanomaterials for Phototherapy of Cancer [J]. Chemical Research in Chinese Universities, 2020, 36(2): 237-242.
[11]	TANG Xu, DU Zhijun, WU Guojie, HAN Fushe. Synthesis of P-Stereogenic Diarylphosphinamides as Novel Inhibitors of Melanoma [J]. Chemical Research in Chinese Universities, 2019, 35(5): 812-816.
[12]	MA Junjie, HUANG Kun, NI Xin, CHEN Roufen, XU Boxuan, WANG Cuifang. Design, Synthesis, Biological Activity and Molecular Docking Study of Coumarin Derivatives Bearing 2-Methyl b iphenyl Moiety [J]. Chemical Research in Chinese Universities, 2019, 35(3): 410-417.
[13]	GUAN Shuwen, YU Xiaoxuan, LI Junyang, XU Heng, HAN Wenzhao, SHI Guannan, XU Jia, WANG Liping. Delivery of Survivin siRNA Using Cationic Diphenylalanine Vesicles [J]. Chemical Research in Chinese Universities, 2019, 35(3): 434-439.
[14]	JIANG Zhenlei, LIU Jie, ZHOU Fan, ZHANG Jianrun, LIU Zhijun, ZHANG Chao, CHEN Heru. One-step Construction of Xanthone Scaffold Assisted by Microwave Irradiation to Optimize the Synthesis of DMXAA [J]. Chemical Research in Chinese Universities, 2018, 34(6): 918-922.
[15]	JIN Bo, TAO Ye, YANG Hongliang. Synthesis, Biological Evaluation and Molecular Docking of Novel Phenylpyrimidine Derivatives as Potential Anticancer Agents [J]. Chemical Research in Chinese Universities, 2018, 34(6): 912-917.

Lentivirus-mediated RNA Interference and Over-expression of CDK2AP1 CDNA Regulate CDK2AP1 Expression in Human Lung Cancer A549 Cells

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments