Preparation of pH-Responsive Doxorubicin Nanocapsules by Combining High-gravity Antisolvent Precipitation with In-situ Polymerization for Intracellular Anticancer Drug Delivery

doi:10.1007/s40242-020-0007-4

Chemical Research in Chinese Universities ›› 2020, Vol. 36 ›› Issue (5): 927-933.doi: 10.1007/s40242-020-0007-4

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Preparation of pH-Responsive Doxorubicin Nanocapsules by Combining High-gravity Antisolvent Precipitation with In-situ Polymerization for Intracellular Anticancer Drug Delivery

LIU Jie¹, CHEN Bo², ZHANG Jianjun¹

1. College of Chemical Engineering, Beijing University of Chemical Technology, Beijing 100029, P. R. China;
2. State Key Laboratory of NBC Protection for Civilian, Beijing 102205, P. R. China

Received:2020-01-17 Revised:2020-02-19 Online:2020-10-01 Published:2020-10-01
Contact: ZHANG Jianjun, CHEN Bo E-mail:zhangjj@mail.buct.edu.cn;NBC-BoChen@163.com
Supported by:
Supported by the National Natural Science Foundation of China(Nos.21476018, 21622601) and the National Key R&D Program of China(No.2015CB932101).

Abstract

Abstract: Owing to the low pH value in tumor and cancer cells, drug delivery systems based on pH-responsive polymer nanocarriers have been extensively explored for anticancer chemotherapy. Herein, we developed a pH-responsive doxorubicin(DOX) nanocapsule(named as DNanoCapsule) prepared by combining in-situ polymerization technique with high-gravity antisolvent precipitation technique through an amphiphilic polymerized surface ligand. DNanoCapsules show an obvious spherical core-shell structure with a single DOX nanoparticle encapsulated in the polymer layer. Dissolution rate studies prove that the DNanoCapsules have robust drug-release profiles under acidic environments due to the division of the pH-sensitive cross-linker, which triggers the collapse of the polymer layer. The in vitro investigations demonstrated that the DNanoCapsules exhibited high cellular uptake efficiency and cytotoxicity for both HeLa and MCF-7 cancer cells. Therefore, this work may provide a promising strategy to design and develop various stimuli-responsive drug nanocapsules for the treatment of cancer or other diseases.

Key words: pH-Responsive, Nanocapsule, In-situ polymerization, High-gravity antisolvent precipitation, Anticancer chemotherapy

LIU Jie, CHEN Bo, ZHANG Jianjun. Preparation of pH-Responsive Doxorubicin Nanocapsules by Combining High-gravity Antisolvent Precipitation with In-situ Polymerization for Intracellular Anticancer Drug Delivery[J]. Chemical Research in Chinese Universities, 2020, 36(5): 927-933.

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Preparation of pH-Responsive Doxorubicin Nanocapsules by Combining High-gravity Antisolvent Precipitation with In-situ Polymerization for Intracellular Anticancer Drug Delivery

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