AI-2 Key Enzyme S-Ribosylhomocysteinase from Strain Klebsiella pneumoniae CICC 10011 Producing 2,3-Butanediol

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (2): 273-276.

• Articles • Previous Articles Next Articles

AI-2 Key Enzyme S-Ribosylhomocysteinase from Strain Klebsiella pneumoniae CICC 10011 Producing 2,3-Butanediol

DAI Jian-ying*, ZHANG Li-fu and XIU Zhi-long*

School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024,?P. R. China

Received:2010-04-13 Revised:2010-07-28 Online:2011-03-25 Published:2011-03-09
Contact: DAI Jian-ying and XIU Zhi-long E-mail:jydai@dlut.edu.cn; zhlxiu@dlut.edu.cn
Supported by:
Supported by the National High-Tech Research and Development Program of China(No.2009AA05Z443).

Abstract

Abstract: S-Ribosylhomocysteinase(LuxS) is the key enzyme in the synthetic pathway of a quorum sensing autoinducer AI-2. LuxS from a 2,3-butanediol produced strain Klebisella pneumoniae CICC 10011 was cloned and characterized. The luxS gene is composed of 540 bp with 172 amino acids encoded. The Km value for S-ribosylhomo- cysteine(SRH) was (27?1) ?mol/L, kcat was (0.112?0.004) s–1 and kcat/Km was 4.4?103 L?mol–1? s–1 at 25 ?C. LuxS was activated by divalent metal ions, the highest activity was detected with Co2+ form, followed by Mg2+, Ba2+, Mn2+, Fe2+ and Ca2+, and activation constant for Co2+ is (16?2) ?mol/L.

Key words: S-Ribosylhomocysteinase(LuxS), Kinetics, Characterization, Klebsiella pneumoniae

DAI Jian-ying*, ZHANG Li-fu and XIU Zhi-long*. AI-2 Key Enzyme S-Ribosylhomocysteinase from Strain Klebsiella pneumoniae CICC 10011 Producing 2,3-Butanediol[J]. Chemical Research in Chinese Universities, 2011, 27(2): 273-276.

[1]	LIU Zailun, SUN Like, ZHANG Qitao, TENG Zhenyuan, SUN Hongli, SU Chenliang. TiO₂-supported Single-atom Catalysts: Synthesis, Structure, and Application [J]. Chemical Research in Chinese Universities, 2022, 38(5): 1123-1138.
[2]	WANG Lu, WANG Dong. Two-dimensional Covalent Organic Frameworks: Tessellation by Synthetic Art [J]. Chemical Research in Chinese Universities, 2022, 38(2): 265-274.
[3]	ZHANG Luwei, LIU Jingyi, BAI Ling, WANG Ning. Diffusion Kinetics Study of Lithium Ion in the Graphdiyne Based Electrode [J]. Chemical Research in Chinese Universities, 2021, 37(6): 1289-1295.
[4]	LI Ying, PAN Bo, MIAO Hongyan, XU Humin, LIU Xuefeng, SHI Gang. Single and Binary Dye Adsorption of Methylene Blue and Methyl Orange in Alcohol Aqueous Solution via Rice Husk Based Activated Carbon: Kinetics and Equilibrium Studies [J]. Chemical Research in Chinese Universities, 2020, 36(6): 1272-1278.
[5]	PEI Zhibin, LIU Yun, SUN Da, ZHU Zixuan, WANG Gongming. Phosphorene: a Potential 2D Material for Highly Efficient Polysulfide Trapping and Conversion [J]. Chemical Research in Chinese Universities, 2020, 36(4): 631-639.
[6]	PAN Lu, DONG Jinyang, YI Ding, YANG Yijun, WANG Xi. Recent Advances in Atomic-scale Storage Mechanism Studies of Two-dimensional Nanomaterials for Rechargeable Batteries Beyond Li-ion [J]. Chemical Research in Chinese Universities, 2020, 36(4): 560-583.
[7]	WU Yi, JING Runan, JIA Guihua, WANG Yixiao, YU Tianmiao, ZHANG Weidong. Measurement of Free and Glucuronidated Cardamonin in Rat Plasma and Bile Using UPLC-MS/MS and Its Application to a Pharmacokinetic and Bile Excretion Study [J]. Chemical Research in Chinese Universities, 2019, 35(6): 962-966.
[8]	Tien V. PHAM. Theoretical Investigation on Mechanism, Thermochemistry, and Kinetics of the Gas-phase Reaction of 2-Propargyl Radical with Formaldehyde [J]. Chemical Research in Chinese Universities, 2019, 35(5): 884-891.
[9]	LIU Ke, XU Yi, YU Hongmei. Research on Ice Crystal Growth Inside the Vitrified Vs55 with Magnetic Nanoparticles During Devitrification by Cryomicroscopy [J]. Chemical Research in Chinese Universities, 2019, 35(3): 542-548.
[10]	QIAO Yaping, YUAN Xiaoyan, ZHAO Yunhui, REN Lixia. Ceiling Degree of Polymerization for Brush Polymers Prepared via ROMP of Poly(tert-Butyl Acrylate) Macromonomers [J]. Chemical Research in Chinese Universities, 2018, 34(5): 828-832.
[11]	CHENG Kai, YANG Wei, WANG Hui, ZHOU Jie, WU Shengji, YU Tianming, PAN Jianbo. Effect of Cu(Ⅱ) on Degradation and Decolorization of Rhodamine B in Subcritical Water [J]. Chemical Research in Chinese Universities, 2017, 33(4): 643-647.
[12]	SEYFI Sara, AZADMEHR Amir Reza, MAGHSOUDI Abbas. Comparative and Competitive Adsorption of Cu (II) and Cd (II) Using Scoria: Equilibrium, Kinetic and Thermodynamic Studies [J]. Chemical Research in Chinese Universities, 2017, 33(3): 471-478.
[13]	LU Min, CHANG Yanwei, CHEN Long. Kinetics of Desilification Pretreatment from High-aluminium Coal Fly Ash in Alkaline Medium Under Pressure [J]. Chemical Research in Chinese Universities, 2017, 33(2): 282-286.
[14]	GU Liqiang, QIU Jianhui, SAKAI Eiichi. Effect of DOPO-containing Flame Retardants on Poly(lactic acid): Non-flammability, Mechanical Properties and Thermal Behaviors [J]. Chemical Research in Chinese Universities, 2017, 33(1): 143-149.
[15]	ZHANG Jihua, LI Jie, LIU Mingjie, ZHAO Yunfeng, WANG Shutao. Thermal Decomposition Kinetics and Mechanism of Low-temperature Hydrogenated Acrylonitrile Butadiene Rubber Composites with Sodium Methacrylate [J]. Chemical Research in Chinese Universities, 2016, 32(6): 1045-1051.

AI-2 Key Enzyme S-Ribosylhomocysteinase from Strain Klebsiella pneumoniae CICC 10011 Producing 2,3-Butanediol

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments