CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (2): 258-263.

• Articles • Previous Articles     Next Articles

Imprinted Human Serum Albumin with Antioxidant Activity

SHEN Na1, YAN Fei1,2, GUO Yi1, LÜ Shao-wu1, GONG Ping-sheng1, XU Ya-wei1, YAN Gang-lin1*, MU Ying1,2* and LUO Gui-min1   

  1. 1. Key Laboratory of Molecular Enzymology and Engineering, Ministry of Education, College of Life Science, Jilin University, Changchun 130012, P. R. China;
    2. State Key Laboratory of Industrial Control Technology, Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, Zhejiang University, Hangzhou 310058, P. R. China
  • Received:2010-10-08 Revised:2010-11-11 Online:2011-03-25 Published:2011-03-09
  • Contact: YAN Gang-lin, MU Ying E-mail:ygl@jlu.edu.cn; ymu100@yahoo.com.cn
  • Supported by:

    Supported by the National Basic Research Program of China(No. 2007CB714503), the National Natural Science Foundation of China(No.30970608), the Applicative Technological Project of Bureau of Science and Technology of Changchun City, China (No.2009045), the Development and Planning Major Program of Jilin Provincial Science and Technology Department, China (No.20100948), the Innovation Method Fund of China(No.2008IM040800) and the Open Project of State Key Laboratory of Supramolecular Structure and Materials of China(No.SKLSSM200915).

PDF (PC)

Cited

28

Abstract: In order to create a new mimic of glutathione peroxidase(GPx), bioimprinting was used to generate glutathione(GSH) binding site and chemical modification was used to incorporate catalytic group selenocystine(Sec). Human serum albumin(HSA) and S-substituted dinitrophenyl glutathione(GSH-S-DNP) were chosen as the imprinted matrix and imprinting template, respectively, to generate a GSH-imprinted protein(GSH-HSA) by bioimprinting. Sec was incorporated into the GSH-HSA by chemical modification to give a new GPx mimic(Se-GSH-HSA). Se-GSH-HSA displayed considerably higher GPx activity than non-printed HSA(Se-HSA). The enzymic properties and kinetics of Se-GSH-HSA were studied. Moreover, Se-GSH-HSA was confirmed to have stronger antioxidant ability to protect mitochondria against oxidative damage with ferrous sulfate/ascorbate-induced mitochondria damage model, indicating that Se-GSH-HSA has potential application in medicine.

Key words: Antioxidant, Enzyme mimic, Glutathione peroxidase, Molecular imprinting

CLC Number: