Study on the Interaction and Properties of Cucurbit[8]uril with Oroxin B

doi:10.1007/s40242-020-0005-6

Abstract

Abstract: The interaction between cucurbit[8]uril(Q[8]) and oroxin B(ORB) was investigated by UV-visible(UV-Vis) spectroscopy, isothermal titration calorimetry(ITC), mass spectrum(MS) and nuclear magnetic resonance(NMR) spectroscopy. The results showed that ORB formed a 2:1 inclusion complex with Q[8] with a binding constant of 8.266×10⁵ L²·mol^-2. ORB had good scavenging ability for 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS) free radicals(IC₅₀=5.74 μmol/L) and the addition of Q[8] did not significantly affect the antioxidant activity of ORB(IC₅₀=5.76 μmol/L). A phase solubility experiment revealed a 1.86-fold increase in the solubility of ORB when c(Q[8])=100 μmol/L. In vitro drug release experiments showed that the release rate for ORB@Q[8] complex was lower than that of ORB in artificial intestinal juice, and higher than that of ORB in artificial gastric juice.

Key words: Cucurbit[8]uril, Oroxin B, Inclusion complex, Solubility, Antioxidant activity

XIE Jun, ZENG Zhishu, TAO Zhu, ZHANG Qianjun. Study on the Interaction and Properties of Cucurbit[8]uril with Oroxin B[J]. Chemical Research in Chinese Universities, 2020, 36(5): 804-809.

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