CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (2): 241-244.

• Articles • Previous Articles     Next Articles

Inhibitory Effects of Natural Compound Alantolactone on Human Non-small Cell Lung Cancer A549 Cells

ZONG Min-ru1, ZHAO Ying-hao2, ZHANG Kun2, YANG Long-fei2, ZHENG Yong-chen2 and HE Cheng-yan1*   

  1. 1. China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China;
    2. The Second Hospital of Jilin University, Changchun 130041, P. R. China
  • Received:2010-12-30 Revised:2011-01-18 Online:2011-03-25 Published:2011-03-09
  • Contact: HE Cheng-yan E-mail:chengyanhe469@vip.sina.com
  • Supported by:

    Supported by the Natural Science Foundation of Jilin Province, China(No.20090461).

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Abstract: Alantolactone is a natural compound identified from the roots of Inula helenium L. that has multiple bio-activities. We examined its inhibitory effects on human non-small cell lung cancer(NSCLC) A549 cells. The antiproliferative effect of alantolactone on A549 cells was investigated via MTT[3′-(4,5dimethylthiazol-2-yl)-2,5-   diphenyl tetrazolium bromide] assay and its apoptosis-inducing effect was determined by Hoechst staining and flow cytometry. We found that alantolactone significantly inhibited the proliferation of A549 cells and induced morphological changes typical for apoptosis. Flow cytometry analysis indicates dose-dependent cell cycle retardation at G0/G1 and S stages. The results indicate that alantolactone could be an attractive small-molecular natural compound for further development as a therapeutic drug against NSCLC.

Key words: Alantolactone, Non-small cell lung cancer(NSCLC), A549, Proliferation, Apoptosis