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Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (1): 70-74.

• Articles • Previous Articles     Next Articles

MicroRNA-mediated Silencing of RhoC Inhibits Tumor Invasion and Increases Chemosensitivity to Paclitaxel in SKOV3 Cells in vitro

PAN Ying, DU Zhen-wu, LENG Wei-chun, ZHOU Jia-wen, WANG Ying-jian, SHENG Min-jia, WANG Jun-rong and ZHANG Gui-zhen*   

  1. China-Japan Union Hospital of Jilin University, Changchun 130031, P. R. China
  • Received:2010-11-01 Revised:2010-12-10 Online:2011-01-25 Published:2011-01-04
  • Contact: ZHANG Gui-zhen E-mail:zhangguizhenjlu@yahoo.com
  • Supported by:

    Supported by the Program of Jilin Provincial Science & Technology Department, China(No.20060415-3).

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Abstract: RhoC is a member of the Ras-homologous family of genes which are implicated in tumorigenesis and tumor progression. Up-regulation of RhoC is associated with tumor progression in ovarian carcinoma and RhoC is significantly correlated with the invasive capability of ovarian cancer cell lines in vitro. We developed a system   that blocks RhoC in the human ovarian cancer SKOV3 cells using specific MicroRNA(miRNA) interference. By transfecting  SKOV3 cells with the plasmid vector to express specific MiRNA that targets human RhoC, we were able to establish a stable clone in which RhoC expression was significantly downregulated. This resulted in the decreased invasive potential of SKOV3 cells as well as increased chemosensitivity to paclitaxel. RhoC involves in  invasion and chemosensitivity of SKOV3, indicating that RhoC may be a promising therapeutic target for ovarian cancer.

Key words: RhoC gene, miRNA, SKOV3 cell, Cell invasion, Paclitaxel