Aquaporin-3 Deletion Reduces Glycerol and ATP Content in Mouse Sciatic Nerve

Chemical Research in Chinese Universities ›› 2010, Vol. 26 ›› Issue (6): 955-957.

• Articles • Previous Articles Next Articles

Aquaporin-3 Deletion Reduces Glycerol and ATP Content in Mouse Sciatic Nerve

ZHANG Di¹, GUO Lei¹, ZHU Na¹, SU Wei-heng¹, GUAN Xin-gang¹, YI Fei¹, HAO Feng¹, XIAO Ying-hong¹, LIU Jia², LIU Tian-zhou² and MA Tong-hui^1,2*

1. Membrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, P. R. China;
2. The Second Hospital of Jilin University, Changchun 130041, P. R. China

Received:2010-06-07 Revised:2010-07-09 Online:2010-11-25 Published:2010-12-01
Contact: MA Tong-hui E-mail:math108@gmail.com
About author:MA Tong-hui. E-mail: math108@gmail.com
Supported by:
Supported by the National Basic Research Program of China(No.2009CB521908), the National Natural Science Foundation of China(Nos.30470405, 30570864, 30670477 and 30770493) and the National Natural Science Fund for Distinguished Young Scholars of China(No.30325011).

Abstract

Abstract: We reported the expression and function of aquaglyceroporin AQP3 in mouse peripheral nervous system. AQP3 mRNA was identified in freshly isolated mouse sciatic nerve by RT-PCR. Immunofluorescence using AQP3 antibody localized the protein expression in the myelin sheathe of sciatic nerve fibers. Although primary morpholo- gical evaluation of sciatic nerves from AQP3-knockout and wildtype mice revealed no significant difference, biochemical analysis demonstrated remarkably decreased glycerol and ATP contents in freshly isolated AQP3-knockout sciatic nerve. The study indicates an important role of facilitated glycerol transport mechanism in peripheral nerve energy metabolism.

Key words: Aquaporin, Sciatic nerve myelin, Glycerol, ATP

ZHANG Di, GUO Lei, ZHU Na, SU Wei-heng, GUAN Xin-gang, YI Fei, HAO Feng, XIAO Ying-hong, LIU Jia, LIU Tian-zhou and MA Tong-hui*. Aquaporin-3 Deletion Reduces Glycerol and ATP Content in Mouse Sciatic Nerve[J]. Chemical Research in Chinese Universities, 2010, 26(6): 955-957.

[1]	HE Xi, YANG Liu, LIU Xintong, WEI Wei, SHI Ce, LI Baoquan, LI Jiang. Ginsenoside Rb1 Upregulating AQP5 Protein Expression and Alleviating Salivary Secretion Impairment in Ovariectomized Sjögren's Syndrome Mice [J]. Chemical Research in Chinese Universities, 2019, 35(3): 418-426.
[2]	WEI Wei, HE Xi, LIU Xintong, LAN Chuanjian, LI Jiang. Estradiol Induced Estrogen Receptor-mediated Transcription and Expression of Aquaporin5 [J]. Chemical Research in Chinese Universities, 2019, 35(2): 239-244.
[3]	WEI Wei, ZHAO Chaoyue, LIU Sitong, JIAO Xiaofei, LIU Xintong, LAN Chuanjian, LI Jiang. Expression of Phytoestrogens in pGL2/AQP1 Promoter Reporter System [J]. Chemical Research in Chinese Universities, 2018, 34(6): 929-933.
[4]	LIU Zhihui, LU Yadong, FENG Wei, YANG Junxing, GAO Shang, SONG Lijie, WANG Yao, WANG Bowei. Synthesis and Properties of PGS-Li Scaffold [J]. Chemical Research in Chinese Universities, 2018, 34(4): 670-675.
[5]	LIANG Guangping, CHUNG Tseyu, GUO Jinhua, ZHANG Rongrong, XÜ Wei, TZEN Jason T. C., JIANG Renwang. Novel Cinobufagin Oxime Ether Derivatives as Potential Na⁺/K⁺-ATPase Inhibitors: Synthesis, Biological Screening and Molecular Docking [J]. Chemical Research in Chinese Universities, 2017, 33(3): 378-383.
[6]	LIU Linlin, ZHAO Dongxia, YANG Zhongzhi. Aggregation of Glycerol Induced by Carbon Nanotubes in Aqueous Solution and Its Influencing Factors [J]. Chemical Research in Chinese Universities, 2015, 31(5): 878-884.
[7]	ZHENG Kun, YANG Hong, ZHAO Xiaofei, JIANG Yang, SUN Chuantao, YANG Jia'an. Probe Staurosporine Drug Binding Site on Protein Kinase by PFSC [J]. Chemical Research in Chinese Universities, 2014, 30(4): 644-649.
[8]	XU Si-yu, ZHAO Feng-qi, YI Jian-hua, GAO Hong-xu, SHAO Zi-qiang, HAO Hai-xia, HU Rong-zu, PEI Qing. Thermal Behavior and Thermal Safety of Nitrate Glycerol Ether Cellulose [J]. Chemical Research in Chinese Universities, 2012, 28(3): 516-519.
[9]	ZHANG Ai-li, LI Jiang, WANG Yan-qing, ZAKNROU Zohra, MA Tong-hui and LI X. Aquaporin 1 Facilitated Hepatocellular Carcinoma SMMC7221 Cell Migration Associated with Water Permeability [J]. Chemical Research in Chinese Universities, 2011, 27(1): 99-103.
[10]	LI Jiang, ZHANG Ai-li, TONG Qian, ZHANG Jun-fang, LI Hai-jing, ZHAO Bing, .... Expression and Emmprin Regulated Function of Aquaporin1 in SMMC7221 Cells [J]. Chemical Research in Chinese Universities, 2010, 26(4): 578-582.
[11]	ZHANG Hong-guo, XIAO Ying-hong, YANG Long-fei, SU Wei-heng, GUAN Xin-gang, .... Altered Taurine Release in Hypothalamus of Aquaporin-4 Knockout Mice [J]. Chemical Research in Chinese Universities, 2010, 26(3): 406-408.
[12]	ZHU Na, QIAO Ying, ZHANG Di, WANG Wen-juan, LIU Jia, LIU Tian-zhou, .... Remarkably Decreased CD11b Positive Splenocytes in Aquaporin 3-Null Mice [J]. Chemical Research in Chinese Universities, 2010, 26(2): 217-220.
[13]	LI Jiang*, YANG Nan-yang, GUAN Xin-gang, ZHANG Shu-zhi, ZHANG Yan,....... GST Fusion Protein Based Specific Polyclonal Antibody Preparation of Mouse Aquaporin 1 [J]. Chemical Research in Chinese Universities, 2009, 25(4): 500-505.
[14]	LU Xiao-ming , PANG Xiao-li , LI Zhi-qiang , WANG Ting , WANG Zhen , YE Chao-hui . Crystal Structure, Nano-particle Morphology and Interaction with ATP of [NH₃CH₂CH(NH₂)CH₃]₂ [ M(VI)O²(OC₆H₄O)₂](M=Mo_0.6W₀[J]. Chemical Research in Chinese Universities, 2007, 23(6): 635-640.
[15]	WU Qing-tian, , MA Qing-jie , HE Cheng-yan , WANG Cai-xia , GAO Shi , HOU Xia , MA Tong-hui . Reduced Bone Mineral Density and Bone Metabolism in Aquaporin-1 Knockout Mice [J]. Chemical Research in Chinese Universities, 2007, 23(3): 297-299.

Aquaporin-3 Deletion Reduces Glycerol and ATP Content in Mouse Sciatic Nerve

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments