CRCU

Chemical Research in Chinese Universities ›› 2018, Vol. 34 ›› Issue (6): 929-933.doi: 10.1007/s40242-018-8139-5

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Expression of Phytoestrogens in pGL2/AQP1 Promoter Reporter System

WEI Wei1, ZHAO Chaoyue1,2, LIU Sitong3, JIAO Xiaofei1, LIU Xintong1, LAN Chuanjian1, LI Jiang1   

  1. 1. Dental Hospital of Jilin University, Changchun 130021, P. R. China;
    2. The First Hospital of Harbin, Harbin 150010, P. R. China;
    3. Edmond H. Fischer Signal Transduction Laboratory, School of Life Sciences, Jilin University, Changchun 130012, P. R. China
  • Received:2018-04-28 Online:2018-12-01 Published:2018-09-29
  • Contact: LI Jiang E-mail:ljiang@jlu.edu.cn
  • Supported by:
    Supported by the Fund of Science and Technology Department of Jilin Province, China(No.20170204009YY), the Fund of Changchun Science & Technology Department, China(No.17YJ001), the Foundation of Ministry of Science and Technology, China(No.2016YFE0128500) and the Project of the Development and Reform Commission of Jilin Province, China (No.2016C048-3).

Abstract: In this study, we amplified aquaporin 1(AQP1) promoter sequence with polymerase chain reaction(PCR), then AQP1 promoter fragment and pGL2 basic vector were linked to create an artificial pGL2/AQP1 promoter reporter system. A certain concentration of 17β-estradiol(E2) activated pGL2/AQP1p, which demonstrated the pGL2/AQP1p transcriptional system effective. The pGL2/AQP1 promoter reporter system was applied to evaluate the activate effect on AQP1 of different kinds of phytoestrogens. Dual luciferase reporter gene activity assay showed that a certain concentration phytoestrogens including daidzein and genistein can increase AQP1 promoter transcription activity. In addition, E2, daidzein and genistein can make AQP1 protein endogenous expression level increase and promote the function of water scretion. The result can guide the clinical application to treat the Sjogren's syndrome and provide a necessary molecular tool for the subsequent drug screening.

Key words: Aquaporin 1(AQP1), Sjogren's syndrome, 17β-Estradiol, Phytoestrogen