Structure Analysis of Pectin SB1-1 from the Root of Panax ginseng

Abstract

Abstract: A water-soluble pectin SB_1-1 was isolated and purified from the root of Panax ginseng C. A. Mey. The HPLC analysis indicates that SB_1-1 is homogenous. Its molecular weight was estimated via gel filtration to be 10000. The GC analysis indicated that it contains the monosaccharides of GalA, Gal, Ara and Rha. Their molar ratio is 2.10∶1.00∶0.12∶0.13. Partial hydrolysis with acid, pectinase treatment, periodate oxidation, Smith degradation, methylation analyses, GC/MS analyses and NMR analyses were used for the structure analyses of SB_1-1. The results reveal that SB_1-1 has a lower branched structure. The main chain is composed of GalA and Gal; the inner part is α-1,4-linked-GalA; the border is 1,4-linked-Gal. Some of the 1,4-linked-GalA and 1,4-linked-Gal residues are substituted at O6. On an average, there is one branch for every ten hexose residues. The side chain is composed of 1,6-linked-Gal and 1,3,6-linked-Gal. The nonreduced end is composed of Rha, Ara and Gal. The main glycosidic link of SB_1-1 has an α configuration.

Key words: Panax ginseng C. A. Mey., Pectin, Structure

TIAN Mei-hong, WEI Min, ZHANG Li-ping, LIANG Zhong-yan. Structure Analysis of Pectin SB_1-1 from the Root of Panax ginseng[J]. Chemical Research in Chinese Universities, 2005, 21(3): 306-308.

References

[1] Helms S., Altern. Med. Rev., 2004, 9, 259 [2] Lee Y. S., Chung I. S., Lee I. R., et al., Anticancer Res., 1997, 17, 323 [3] Lim T. S., Na K., Choi E. M., et al., J. Med. Food, 2004, 7, 1 [4] Park K. M., Kim Y. S., Jeong T. C., et al., Planta Med., 2001, 67, 122 [5] Lee J. H., Lee J. S., Chung M. S., et al., Planta Med., 2004, 70, 566 [6] Gao Q. P., Kiyohara H., Yamada H., Carbohydr. Res., 1990, 196, 111 [7] Lim D. S., Bae K. G., Jung I. S., et al., J. Infect., 2002, 45, 32 [8] Belogortseva N. I., Yoon J. Y., Kim K. H., Planta Med., 2000, 66, 217 [9] Tian M. H., Wei M., Zhang L. P., et al., J. Northeast Normal Univ., 2005, 37, 93 [10] Wei M., Zhang L. P., Liang Z. Y., et al., J. Northeast Normal Univ., 2003, 35, 74 [11] Zhang W. J., Ed., Research Techniques of Glycoconjugates in Biochem., Zhejiang University Press, Hangzhou, 1999, 140 [12] Liang Z. Y., Zhang Y. S., Chem. J. Chinese Universities., 1983, 4(5), 364 [13] Ciucanu I., Kerek F., Carbohydr. Res., 1984, 131, 209 [14] Lindberg B., Methods in Enzymology, Academic Press, New York, 1972, 28, 178 [15] Taylor R. L., Conrad H. E., Biochemistry, 1972, 11, 1383 [16] Kitamura S., Hori T., Kurita K., et al., Carbohydr. Res., 1994, 263, 111 [17] Biermann C. J., McGinnis G. D., Ed., Analysis of Carbohydrates by GLC and MS, CRC Press, Florida, 1989, 158 [18] Jansson P. E., Chem. Commun. Univ. Stockholm, 1976, 8, 1 [19] Bjrndal H., Lindberg B., Svensson S., Carbohydr. Res., 1967, 5, 433 [20] Basak R. K., Mandal P. K., Mukherjee A. K., Carbohydr. Res., 1981, 97, 315 [21] Jansson P. E., Lindberg B., Sandford P. A., Carbohydr. Res., 1983, 124, 135 [22] Yamada H., Kiyohara H., Cyong J. C., et al., Carbohydr. Res., 1987, 159, 275 [23] Zhang W. J., Ed., Research Techniques of Glycoconjugates in Biochemistry, Zhejiang University Press, Hangzhou, 1999, 242

Structure Analysis of Pectin SB_1-1 from the Root of Panax ginseng

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