Improvement of Properties of Pseudomonas sp. Lipase in Resolution of 2-Octanol

Chemical Research in Chinese Universities ›› 2003, Vol. 19 ›› Issue (4): 446-449.

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Improvement of Properties of Pseudomonas sp. Lipase in Resolution of 2-Octanol

WANG Zhi¹, QUAN Jing¹, WENG Liang¹, ZHENG Liang-yu¹, LIU Ning², DONG Huan¹, CAO Shu-gui¹

1. Key Laboratory for Molecular Enzymology and Engineering of Educational Ministry;
2. College of Life Science, Jilin University, Changchun 130023, P. R. China

Received:2002-12-05 Online:2003-12-24 Published:2011-08-06
Supported by:
Supported by the National Natural Science Foundation of China(No.20072011 and 20272017).

Abstract

Abstract: A novel preparation method was developed to form a surfactant-lipase complex for the resolution of 2-octanol in a solvent-free system. The E value was improved from 6.64 to 120.2. The lipase modified by the anionic surfactant possessed a low solubility in the solvent-free system, which was beneficial to the recovery and the repeated usage of the lipase. The surfactant-lipase complex maintained a high enantioselectivity after five cycles of usage. The effect of water on both the activity and the enantioselectivity of the lipase has also been investigated; the direct addition of a salt hydrate pair of Na₄P₂O₇·H₂O and Na₄P₂O₇ can dramatically activate the modified enzyme.

Key words: Lipase, Surfactant, Salt hydrate pair, Esterification, Enantioselectivity

WANG Zhi, QUAN Jing, WENG Liang, ZHENG Liang-yu, LIU Ning, DONG Huan, CAO Shu-gui. Improvement of Properties of Pseudomonas sp. Lipase in Resolution of 2-Octanol[J]. Chemical Research in Chinese Universities, 2003, 19(4): 446-449.

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Improvement of Properties of Pseudomonas sp. Lipase in Resolution of 2-Octanol

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