Detection of Catecholic Compounds withField-ampilfied CZE-Amperometry

Abstract

Abstract: On-column sample stacking technique has been used extensively in capillary zone electrophoresis (CZE) to enhance the sensitivity of the measurements with UV-Vis detection. However, the detection limits for CZE-separation are still limit- ed due to the poor sensitivity of a UV-Vis detector. We incorporated field-amplified sample stacking technique into CZE with amperometric detection. Compared with the conventional electromigration injection, a negative peak of water was observed and a great enhancement of signals was obtained. The results in phosphate buffer with sodium dodecyl sulfate (SDS ) and 2-N-(morpholino) ethane sulphonic acid ( MES ) buffer were compared. Subnanomolar concentration limits for cate- cholamine neurotransmitters (dopamine, norepinephrine, epinephriine) and isopro- terenol were achieved in the 25 um capillary with two or three orders of magnitude lower than those with amperometric detection reported previously.

Key words: Capillary zone electrophoresis, Amperometric detection, Stacking

HU Shen, PANG Dai-wen, HU Yong-gang, CHENG Jie-ke . Detection of Catecholic Compounds withField-ampilfied CZE-Amperometry[J]. Chemical Research in Chinese Universities, 1997, 13(3): 221-228.

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Detection of Catecholic Compounds withField-ampilfied CZE-Amperometry

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