CRCU

Chemical Research in Chinese Universities ›› 1995, Vol. 11 ›› Issue (4): 279-283.

• Articles •     Next Articles

Tb3+ as Fluorescent Probe in the Study of the Interaction of Other Lanthanide Ions with Trichosanthin

HAO Qiang1, LI Wen-lian2, ZHANG Yan1, YU Gui2, YANG Hong1, ZHAO Xu2, YAO Qi-Zhi3, LI Qing-shan1   

  1. 1. Department of Molecular Biofogy and National Laboratory of enzyme engineering, Jiln University, Changchun, 130023;
    2. Changchun Institute of Physics, Academia Sinica, Changchun, 130023;
    3. Graduate School of Acadrmia, Beijing, 100039
  • Received:1994-12-18 Online:1995-12-24 Published:2011-08-17
  • Supported by:

    Supported by the National Natural Science Foundation of China and Lab. of Excited State Processes, Changchun Institute of Physics. Supported also by National Lab. of Enzyme Engineering, Jilin University.

PDF (PC)

Abstract: The interaction of lanthanide (Ln3+)ions with trchosanthin(TCS)was investi-gated using Tb3+ as fluorescent probe.The metal-binding sites of the protein were probed by means of adding the Tb3+ into the protein solutions.If other Ln3+ ions exist in the Tb3+ protein complex system, they would compete against Tb3+ in the binding sites of the protein, and so the fluorescence intensity of Tb3+ decreased,which is called quenching effect.The quencliing effect is related to the ionic radii of the Ln3+ ions and energy transfer from Tb3+ protein to Ln3+ ions.Based on the ex-perimental results,a schematic of the energy transfer from protein-Tb3+ complex to protein-Ln3+ complex is suggested.

Key words: Trichosanthin, Energy transfer, Protein-Ln3+ complex