Dark-vacuole Bodies Studying by High-resolution Label-free Microscopy Assisted with Fluorescence Technology

doi:10.1007/s40242-024-3271-x

Chemical Research in Chinese Universities ›› 2024, Vol. 40 ›› Issue (6): 978-986.doi: 10.1007/s40242-024-3271-x

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Dark-vacuole Bodies Studying by High-resolution Label-free Microscopy Assisted with Fluorescence Technology

LIU Xiangyu^1,2, ZHANG Jinrui¹, XU Haijiao¹, SHAO Lina¹, WANG Hongda^1,2,3

1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
2. School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei 230026, P. R. China;
3. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, P. R. China

Received:2023-12-08 Online:2024-12-01 Published:2024-10-26
Contact: WANG Hongda,hdwang@ciac.ac.cn;SHAO Lina,lnshao@ciac.ac.cn E-mail:hdwang@ciac.ac.cn;lnshao@ciac.ac.cn
Supported by:
This work was supported by the Scientific Instrument Developing Project of the Chinese Academy of Sciences (No. ZDKYYQ20220005) and the National Natural Science Foundation of China (Nos. 22150003, 21727816, and 21721003).

Abstract

Abstract: The intracellular logistics system, consisting of vesicles, plays a crucial role in cellular transport. However, there is a lack of research on the types and functions of intracellular vesicles, and new technologies are needed for further investigation. Recently, researchers have discovered a new subcellular structure known as Dark-vacuole bodies. The composition, function, and potential synergy with other organelles of these Dark-vacuole bodies remain unclear. In this study, we utilized the high-resolution label-free Fourier ptychographic microscopy, developed by our research group, along with fluorescence confocal technology, to study and analyze Dark-vacuole bodies. Our findings provide evidence of the influence of Dark-vacuole bodies on the morphology, distribution, movement, and cell cycle of living cells. Specifically, we analyzed the effects of drug induced stimulation of lipid drops and endosomes, promotion of cell endocytosis, and induction of cellular senescence on Dark-vacuole bodies. Our results indicate that Dark-vacuole bodies show little correlation with lipid drops and endocytosis vesicles, but are significantly associated with late endosomes. Furthermore, cellular senescence leads to a significant increase in the number and size of Dark-vacuole bodies. This study serves as a foundation for further confirming the nature of Dark-vacuole bodies as new organelles.

Key words: Dark-vacuole body, Subcellular Organelle, Label-free, Fourier ptychographic microscopy (FPM), Living cell imaging

LIU Xiangyu, ZHANG Jinrui, XU Haijiao, SHAO Lina, WANG Hongda. Dark-vacuole Bodies Studying by High-resolution Label-free Microscopy Assisted with Fluorescence Technology[J]. Chemical Research in Chinese Universities, 2024, 40(6): 978-986.

References

[1] Das Gupta A., Krawczynska N., Nelson E. R., Endocrinology, 2021, 162, doi: 10.1210/endocr/bqab133.
[2] Cui L., Li H., Xi Y., Hu Q., Liu H., Fan J., Xiang Y., Zhang X., Shui W., Lai Y., Mol. Biomed., 2022, 3, 29.
[3] Palade G., Science, 1975, 189, 867.
[4] Winters C. M., Hong-Brown L. Q., Chiang H. L., J. Biol. Chem., 2020, 295, 2650.
[5] Ginini L., Billan S., Fridman E., Gil Z., Cells, 2022, 11, doi: 10.3390/ cells11091375.
[6] Xu H., Wang H., Chem. Res. Chinese Universities, 2021, 37, 829.
[7] Verweij F. J., Balaj L., Boulanger C. M., Carter D. R. F., Compeer E. B., D'Angelo G., El Andaloussi S., Goetz J. G., Gross J. C., Hyenne V., Krämer-Albers E. M., Lai C. P., Loyer X., Marki A., Momma S., Nolte-'t Hoen E. N. M., Pegtel D. M., Peinado H., Raposo G., Rilla K., Tahara H., Théry C., van Royen M. E., Vandenbroucke R. E., Wehman A. M., Witwer K., Wu Z., Wubbolts R., van Niel G., Nat. Methods, 2021, 18, 1013.
[8] Dong D., Huang X., Li L., Mao H., Mo Y., Zhang G., Zhang Z., Shen J., Liu W., Wu Z., Liu G., Liu Y., Yang H., Gong Q., Shi K., Chen L., Light Sci. Appl., 2020, 9, 11.
[9] Zheng G., Horstmeyer R., Yang C., Nat Photonics, 2013, 7, 739.
[10] Zheng G., Ou X., Horstmeyer R., Chung J., Yang C., Optics and Photonics News, 2014, 25, 26.
[11] Jensen J. A., Tomov B. G., Haslund L. E., Panduro N. S., Sorensen C. M., IEEE Trans Ultrason Ferroelectr Freq Control, 2023, 70, 708.
[12] Gao P., Pedrini G., Osten W., Opt. Lett., 2013, 38, 1328.
[13] Maire G., Giovannini H., Talneau A., Chaumet P. C., Belkebir K., Sentenac A., Opt. Lett., 2018, 43, 2173.
[14] Ancora D., Di Battista D., Giasafaki G., Psycharakis S. E., Liapis E., Ripoll J., Zacharakis G., Methods, 2018, 136, 81.
[15] Sun M., Chen X., Zhu Y., Li D., Mu Q., Xuan L., Opt. Express, 2019, 27, 24161.
[16] Pan A., Zhang Y., Wen K., Zhou M., Min J., Lei M., Yao B., Opt. Express, 2018, 26, 23119.
[17] Shen Q., Xu R., Wang Z., Zhao T., Zhou Y., Xu Y., Yang Z., Lei M., Meng L., Dang D., Chem. Res. Chinese Universities, 2021, 37, 143.
[18] Sun L., Wang Y., Zhang H., Min C., Zhang Y., Zhang C., Xin Z., Zhu S., Yang Y., Burge R. E., Yuan X., ACS Sens., 2020, 5, 510.
[19] Xing F., Meng G. X., Zhang Q., Pan L. T., Wang P., Liu Z. B., Jiang W. S., Chen Y., Tian J. G., Nano Lett., 2014, 14, 3563.
[20] Marquet P., Rappaz B., Magistretti P. J., Cuche E., Emery Y., Colomb T., Depeursinge C., Opt. Lett., 2005, 30, 468.
[21] Song N., Xiao P., Ma K., Kang M., Zhu W., Huang J., Wang D., Tang B. Z., Chem. Res. Chinese Universities, 2021, 37, 52.
[22] Krahmer N., Guo Y., Wilfling F., Hilger M., Lingrell S., Heger K., Newman H. W., Schmidt-Supprian M., Vance D. E., Mann M., Farese R. V., Jr., Walther T. C., Cell Metab, 2011, 14, 504.
[23] Li S., Pan Y., Teng H., Shan Y., Yang G., Wang H., Chem. Res. Chinese Universities, 2022, 38, 838.
[24] Dufès C., Al Robaian M., Somani S., Ther. Deliv., 2013, 4, 629.
[25] Daniels T. R., Delgado T., Rodriguez J. A., Helguera G., Penichet M. L., Clin. Immunol., 2006, 121, 144.
[26] Xu H., Zhang J., Zhou Y., Zhao G., Cai M., Gao J., Shao L., Shi Y., Li H., Ji H., Zhao Y., Wang H., Research, 2022, 2022, 9835035.
[27] Zhang W., Li J., Suzuki K., Qu J., Wang P., Zhou J., Liu X., Ren R., Xu X., Ocampo A., Yuan T., Yang J., Li Y., Shi L., Guan D., Pan H., Duan S., Ding Z., Li M., Yi F., Bai R., Wang Y., Chen C., Yang F., Li X., Wang Z., Aizawa E., Goebl A., Soligalla R. D., Reddy P., Esteban C. R., Tang F., Liu G. H., Belmonte J. C., Science, 2015, 348, 1160.
[28] Wu Z., Zhang W., Song M., Wang W., Wei G., Li W., Lei J., Huang Y., Sang Y., Chan P., Chen C., Qu J., Suzuki K., Belmonte J. C. I., Liu G. H., Protein Cell, 2018, 9, 333.
[29] Chang B. D., Broude E. V., Dokmanovic M., Zhu H., Ruth A., Xuan Y., Kandel E. S., Lausch E., Christov K., Roninson I. B., Cancer Res., 1999, 59, 3761.
[30] Hayakawa A., Leonard D., Murphy S., Hayes S., Soto M., Fogarty K., Standley C., Bellve K., Lambright D., Mello C., Corvera S., Proceedings of the National Academy of Sciences, 2006, 103, 11928.

Dark-vacuole Bodies Studying by High-resolution Label-free Microscopy Assisted with Fluorescence Technology

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