DNA-silver Nanocluster Binary Probes for Ratiometric Fluorescent Detection of HPV-related DNA

doi:10.1007/s40242-019-9085-6

Chemical Research in Chinese Universities ›› 2019, Vol. 35 ›› Issue (4): 581-585.doi: 10.1007/s40242-019-9085-6

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DNA-silver Nanocluster Binary Probes for Ratiometric Fluorescent Detection of HPV-related DNA

YUAN Yan, LI Shaoyuan, LUO Lei, WANG Qing, FANG Hongmei, HUANG Jin, LIU Jianbo, YANG Xiaohai, WANG Kemin

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, P. R. China

Received:2019-03-27 Revised:2019-05-01 Online:2019-08-01 Published:2019-08-01
Contact: YANG Xiaohai, WANG Kemin E-mail:yangxiaohai@hnu.edu.cn;kmwang@hnu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(Nos.21675047, 21735002, 21521063) and the Key Point Research and Invention Program of Hunan Province, China(No.2017DK2011).

Abstract

Abstract: Herein, we described a ratiometric strategy based on "chameleon" DNA-silver nanoclusters(DNA-AgNCs) fluorescent binary probes. The strategy was applied to detect high-risk human papillomavirus(HPV) DNA sequences, HPV-16. First, DNA-AgNCs were synthesized by a simple reduction method. The obtained nanoprobes showed typical yellow and red fluorescence of AgNCs. Upon the addition of HPV-16 DNA, the yellow fluorescence of AgNCs was reduced greatly, whereas the red fluorescence of AgNCs was increased. The concentration of HPV-16 DNA in the samples was characterized by the ratio of fluorescence intensity at 570 and 630 nm. The ratiometric nanoprobes showed good selectivity for HPV-16 DNA, and the detection limit was 2 nmol/L. In addition, the practical applicability of this strategy was demonstrated by analysing the HPV-16 DNA in human serum, illustrating its potential promise for clinical diagnosis.

Key words: DNA-silver nanocluster, Ratiometric, Binary probe, Human papillomavirus

YUAN Yan, LI Shaoyuan, LUO Lei, WANG Qing, FANG Hongmei, HUANG Jin, LIU Jianbo, YANG Xiaohai, WANG Kemin. DNA-silver Nanocluster Binary Probes for Ratiometric Fluorescent Detection of HPV-related DNA[J]. Chemical Research in Chinese Universities, 2019, 35(4): 581-585.

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DNA-silver Nanocluster Binary Probes for Ratiometric Fluorescent Detection of HPV-related DNA

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