Chemotransformation of Bacterial Cells Without Heat-shock

doi:10.1007/s40242-017-6403-8

Chemical Research in Chinese Universities ›› 2017, Vol. 33 ›› Issue (2): 160-165.doi: 10.1007/s40242-017-6403-8

• Articles • Previous Articles Next Articles

Chemotransformation of Bacterial Cells Without Heat-shock

WANG Sainan, LI Zhenhua, CHEN Zhijun

State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, International Joint Research Laboratory of Nano-micro Architecture Chemistry, Jilin University, Changchun 130012, P. R. China

Received:2016-09-29 Revised:2016-11-18 Online:2017-04-01 Published:2017-01-09
Contact: CHEN Zhijun,E-mail:zchen@jlu.edu.cn E-mail:zchen@jlu.edu.cn
Supported by:
Supported by the National Natural Science Foundation of China(No.21372097) and the Grant from the Center of International Mobility(CIMO) of Finland.

Abstract

Abstract:

Transformation of DNA into microbial cells via heat-shock approach has been well established in the field of molecular biology for decades. Herein we described an unexpected finding that heat-shock may not play an essential role in the transformation process. This observation was verified via UV-Vis and fluorescence spectroscopies, and confocal microscopy images for various DNAs and bacterial cells. The non-heat-shock approach proposed in this study can be a convenient and beneficial modification for DNA transformation, especially for those laboratories lacking ice machine and heat-shock equipment.

Key words: Heat-shock, Chemotransformation, DNA, Bacteria

WANG Sainan, LI Zhenhua, CHEN Zhijun. Chemotransformation of Bacterial Cells Without Heat-shock[J]. Chemical Research in Chinese Universities, 2017, 33(2): 160-165.

References

[1] Cohen S. N., Chang A. C., HsuL., P. Natl. Acad. Sci. USA, 1972, 69, 2110
[2] Bergmans H. E. N., van Die I. M., Hoekstra W. P. M., J. Bacteriol., 1981, 146, 564
[3] Dower W. J., Miller J. F., Ragsdale C. W., Nucleic Acids Res., 1988, 16, 6127
[4] Madden K., Tolstorukov L., Cregg J., Fungal Biol., 2015, 1, 87
[5] Deeks J., Windmill J., Agbeze-Onuma M., Kalin R. M., Argondizza P., Knapp C. W., Biotechnol. Lett., 2014, 36, 2461
[6] Dityatkin S. Y. A., Iliyashenko B. N., Genetika, 1979, 15, 22
[7] Vincze E., Bowra S., Appl. Environ. Microbiol., 2006, 72, 2290
[8] Kawata Y., Yano S., Kojima H., Biosci. Biotech. Biochem., 2003, 67, 1179
[9] Fregel R., Rodrí guez V., Cabrera V. M., Lett. Appl. Microbiol., 2008, 46, 498
[10] Smith F. D., Harpending P. R., Sanford J. C., J. Gen. Microbiol., 1992, 138, 239
[11] Yoshida N., Ide K., Appl. Microbiol. Biot., 2008, 80, 813
[12] Yoshida N., Sato M., Appl. Microbiol. Biot., 2009, 83, 791
[13] Mandel M., Higa A., J. Mol. Biol., 1970, 53, 159
[14] Hanahan D., J. Mol. Biol., 1983, 166, 557
[15] Li M., Kang H. F., Guo Z. H., Chem. J. Chinese Universities, 2016, 37(7), 1269
[16] Jin L., Yang P., Li Q. S., Chem. Res. Chinese Universities, 1997, 13(3), 201
[17] Duguid J., Bloomfield V. A., Benevides J., Thomas Jr. G. J., Biophys. J., 1993, 65, 1916
[18] Marino T., Mazzuca D., Russo N., Toscano M., Grand A., Int. J. Quantum Chem., 2010, 110, 138
[19] Ross P. D., Scruggs R. L., Biopolymers, 1964, 2, 79
[20] Cosloy S. D., Oishi M., Mol. Gen. Genet., 1973, 124, 1
[21] Norgard M. V., Keem K., Monahan J. J., Gene, 1978, 3, 279
[22] Inoue H., Nojima H., Okayama H., Gene, 1990, 96, 23
[23] Inoue T., Heo W. D., Grimley J. S., Wandless T. J., Meyer T., Nat. Methods, 2005, 2, 415
[24] Hung Y. P., Albeck J. G., Tantama M., Yellen G., Cell Metab., 2011, 14, 545
[25] Vandie I. M., Bergmans H. E. N., Hoekstra W. P. M., J. Gen. Microbiol., 1983, 129, 663470
[26] van der Rest M. E., Lange C., Molenaar D., Appl. Microbiol. Biotechnol., 1999, 52, 541

Chemotransformation of Bacterial Cells Without Heat-shock

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG Qian, LIANG Yuyan, XING Hang. Caging-Decaging Strategies to Realize Spatiotemporal Control of DNAzyme Activity for Biosensing and Bioimaging [J]. Chemical Research in Chinese Universities, 2022, 38(4): 902-911.
[2]	HUANG Qin, LIU Xin, ZHANG Pengge, WU Zhan, ZHAO Zilong. A DNA Nano-train Carrying a Predefined Drug Combination for Cancer Therapy [J]. Chemical Research in Chinese Universities, 2022, 38(4): 928-934.
[3]	PAN Chuanqi, LIU Xiaoxuan, ZHANG Xiang, MAO Feihong, XU Peiyan, ZHU Yuhua, DENG Hongtao, LUO Zhu, SUN Hongwei, ZHANG Lizhi, GUO Yanbing. Fabrication and Excellent Antibacterial Activity of Well-defined CuO/Graphdiyne Nanostructure [J]. Chemical Research in Chinese Universities, 2021, 37(6): 1341-1347.
[4]	YU Qing, HUANG Xuan, ZHANG Tian, WANG Weili, YANG Dongliang, SHAO Jinjun, DONG Xiaochen. Near-infrared Aza-BODIPY Dyes Through Molecular Surgery for Enhanced Photothermal and Photodynamic Antibacterial Therapy [J]. Chemical Research in Chinese Universities, 2021, 37(4): 951-959.
[5]	HAN Lin, WANG Yuang, TANG Wantao, LIU Jianbing, DING Baoquan. Bioimaging Based on Nucleic Acid Nanostructures [J]. Chemical Research in Chinese Universities, 2021, 37(4): 823-828.
[6]	YIN Fangfei, CAO Nan, XIANG Xuelin, FENG Hao, LI Fan, LI Min, XIA Qiang, ZUO Xiaolei. DNA Framework-based Topological Aptamer for Differentiating Subtypes of Hepatocellular Carcinoma Cells [J]. Chemical Research in Chinese Universities, 2021, 37(4): 919-924.
[7]	LIU Zhenyu, DONG Jinyi, PAN Jiahao, ZHOU Chao, FAN Chunhai, WANG Qiangbin. Catalytic DNA Origami-based Chiral Plasmonic Biosensor [J]. Chemical Research in Chinese Universities, 2021, 37(4): 914-918.
[8]	CHU Binbin, WANG Houyu, HE Yao. Fluorescent Silicon-based Nanomaterials Imaging Technology in Diseases [J]. Chemical Research in Chinese Universities, 2021, 37(4): 880-888.
[9]	QIN Mei, LIU Daqing, DAI Zhang, MENG Xin, LIU Guosai, LIU Hao, HUANG Xiaowei, YAN Xu, CHEN Shaojuan. One Step Fabrication and Application of Antibacterial Electrospun Zein/Cinnamon Oil Membrane Wound Dressing via In situ Electrospinning Process [J]. Chemical Research in Chinese Universities, 2021, 37(3): 464-469.
[10]	YAN Jie, WANG Dong, BAI Tian, CHENG Wanli, HAN Guangping, NI Xiaohui, SHI Q. Sheldon. Electrospun PVA Nanofibrous Membranes Reinforced with Silver Nanoparticles Impregnated Cellulosic Fibers: Morphology and Antibacterial Property [J]. Chemical Research in Chinese Universities, 2021, 37(3): 505-511.
[11]	HUANG Libing, LIU Yueqi, SANG Xinyu, SONG Jinghui, HU Ping, LIU Yong. Preparation of Alum-borneol-PVP Drug-loaded Fibers by Electrospinning [J]. Chemical Research in Chinese Universities, 2021, 37(3): 411-418.
[12]	ZHANG Ling, FU Yihong, DING Yue, MENG Jiao, WANG Zhenchao, WANG Peiyi. Antibacterial Activity of Novel 18β-Glycyrrhetinic Hydrazide or Amide Derivatives [J]. Chemical Research in Chinese Universities, 2021, 37(3): 662-667.
[13]	HAO Xueyu, LI Chunjie, WANG Yu, ZHANG Feng, HOU Jingwei, KANG Chunqing, GAO Lianxun. NDI-induced Topological Conversion of Human Telomeric G-Quadruplexes from Hybrid-2 to Parallel Form [J]. Chemical Research in Chinese Universities, 2021, 37(3): 795-800.
[14]	HU Qinyu, WU Jun, CHEN Lulu, LOU Xiaoding, XIA Fan. Recent Development of DNA-modified AIEgen Probes for Biomedical Application [J]. Chemical Research in Chinese Universities, 2021, 37(1): 66-72.
[15]	WANG Lili, LIU Yanan, PENG Xiaoxing, SUN Yuanqing, LIU Xiaohua, LIU Huimin, LIN Quan, SUN Hongchen, YANG Bai, LI Xiangwei. Preparation and Characterization of CaO/ZnO Core-shell Structured Nanoparticles [J]. Chemical Research in Chinese Universities, 2020, 36(5): 970-975.