CRCU

Chemical Research in Chinese Universities ›› 2019, Vol. 35 ›› Issue (5): 782-787.doi: 10.1007/s40242-019-9030-8

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Chemical Fingerprint for Identification and Quality Control of Saccharides in Danhong Injection Based on HPLC-ELSD with Chemometrics

LI Hao1,2, YANG Zhenzhong2, WANG Jiaojiao2, AN Yun3, WANG Chenchen4, GUO Xiaoguang4, WANG Yi2   

  1. 1. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P. R. China;
    2. College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, P. R. China;
    3. Heze Medical College, Heze 274000, P. R. China;
    4. Shandong Danhong Pharmaceutical Co., Ltd., Heze 274000, P. R. China
  • Received:2019-01-29 Online:2019-10-01 Published:2019-09-24
  • Contact: WANG Yi E-mail:zjuwangyi@zju.edu.cn
  • Supported by:
    Supported by the National Key Scientific and Technological Project of China(No.2017ZX09301012), the National Natural Science Foundation of China(No.81822047), and the Fundamental Research Funds for the Central Universities of China(No. 2019XZZX004-16).

Abstract: Chemical fingerprinting has been widely used for the quality control of complex natural products inclu-ding traditional Chinese medicines and botanical drugs. However, there is still lack of appropriate method to monitor the batch-to-batch consistency of hydrophilic constituents, such as saccharides and oligosaccharides. In the present study, we developed a novel approach based on high performance liquid chromatography with evaporative light scattering detector(HPLC-ELSD) for establishing saccharide fingerprinting of Danhong Injection(DHI), which is a widely used botanical drug for treating cardiovascular diseases. Major saccharides in DHI were isolated and four compounds including fructose, glucose and two oligosaccharides were identified. The structures of two novel saccharides named glycerol-1-O-galactfpyranosyl-(1→4)-O-arabinofuranoside, and glycerol-1-O-galactpyranosyl-(1→4)[O-arabinfuranosyl-(1→3)]-O-arabinofuranoside were confirmed by NMR, HR-ESI-MS and GC-MS for the first time. The establishment approach was successfully applied to distinguishing 12 batches of DHI from other botanical drugs with the aid of principle component analysis as well as to evaluating batch-to-batch consistency with the help of calculating similarity of fingerprints. Our findings indicate that the chemical fingerprint of saccharides can be a useful tool for the quality control of complex natural products.

Key words: Danghong injection, Saccharide, Fingerprint, Carthami Flos