Determination of Sex Hormones in Antler Velvet by High Performance Liquid Chromatography Tandem Mass Spectrometry

Chemical Research in Chinese Universities ›› 2012, Vol. 28 ›› Issue (2): 191-194 .

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Determination of Sex Hormones in Antler Velvet by High Performance Liquid Chromatography Tandem Mass Spectrometry

LU Chun-mei^1,3, WANG Ming-tai³, MU Jun³, BAI Yu-ping⁴, DU Jian-shi^1,2, ZHANG Han-qi¹, WANG Jian-wei⁵

1. College of Chemistry, Jilin University, Changchun 130012, P. R. China;
2. China-Japan Union Hospital of Jilin University, Changchun 130021, P. R. China;
3. Jilin Entry-exit Inspection and Quarantine Bureau, Changchun 130061, P. R. China;
4. China FAW Group Corporation R&D Center, Changchun 130011, P. R. China;
5. Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China

Received:2011-08-08 Revised:2011-10-23 Online:2012-03-25 Published:2012-03-08
Supported by:
Supported by the Projects of the General Administration of Quality Supervision, Inspection and Quarantine of China(No. 2007IK157).

Abstract

Abstract: Eighteen sex hormones in antler velvet were determined by high performance liquid chromatography tandem mass spectrometry. The solid phase extraction was applied to eliminating the matrix effect. The experimental conditions were examined and optimized. Under the optimal conditions, the proposed method provides the good linearities and determination limits(0.2―1.0 μg/kg) of the analytes investigated. The recoveries ranging from 72.3% to 149.5% were obtained for the target analytes at two concentration levels. This method was applied to the determination of eighteen sex hormones in different kinds of antler velvet samples and the obtained results are satisfactory. The results indicate that the proposed method is suitable for the determination of sex hormones in antler velvet samples.

Key words: Antler velvet, Liquid chromatography tandem mass spectrometry, Sex hormone, Solid-phase extraction

LU Chun-mei, WANG Ming-tai, MU Jun, BAI Yu-ping, DU Jian-shi, ZHANG Han-qi, WANG Jian-wei. Determination of Sex Hormones in Antler Velvet by High Performance Liquid Chromatography Tandem Mass Spectrometry[J]. Chemical Research in Chinese Universities, 2012, 28(2): 191-194 .

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Determination of Sex Hormones in Antler Velvet by High Performance Liquid Chromatography Tandem Mass Spectrometry

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