Taurine Inhibits Myocardial Fibrosis via PKC-ERK1/2 Signaling Pathways

Chemical Research in Chinese Universities ›› 2012, Vol. 28 ›› Issue (1): 84-90.

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Taurine Inhibits Myocardial Fibrosis via PKC-ERK1/2 Signaling Pathways

WANG Li-ying^1,2, LI Hong¹, YANG Shi-jie^1*

1. Department of Pharmacology, Norman Bethune College of Medicine, Jilin University, Changchun 130021, P. R. China;
2. Department of Physiology, Basic Medicine College, Beihua University, Jilin 132013, P. R. China

Received:2011-08-31 Revised:2011-09-23 Online:2012-01-25 Published:2011-12-27
Contact: YANG Shijie E-mail:jcyaoli@sina.com
Supported by:
Supported by the National Basic Research Program of China(No.2007CB512006) and the National Natural Science Foundation of China(No.30873066/C180102).

Abstract

Abstract: Previous studies have demonstrated the important role of taurine in inhibiting proliferation of myofibroblasts( myoFb) and myocardial fibrosis. However, the underlying mechanisms are unclear. The present study was designed to shed light on this issue through exploring the signal pathways via in vitro experiments. Angiotension Ⅱ (AngⅡ) treatment significantly increased myoFb proliferation and the levels of collagens Ⅰ and Ⅲ(P<0.05), whereas taurine, PKCα(PKC: protein kinase C) specific inhibitor L-threo-dihydro-sphingosine(D4681), ERK1/2 inhibitor (PD98095) abrogated myoFb proliferation and collagen levels(P<0.05, P<0.01, respectively), and increased the G₀/G₁ phase rate and decreased S phase rate. Immunocytochemistry, confocal fluorescence staining and image analysis showed that taurine could inhibit the translocation and expression of p-PKCαin membrane, and then inhibit nuclear translocation and expression of p-ERK1/2. These results have statistically significant differences compared with those of AngⅡ group(P<0.01). Western blot results also show that taurine could inhibit the protein expression of p-PKCα and p-ERK1/2. We used p-PKCα specific inhibitor D4681 in order to elucidate the relationship between p-PKCα and p-ERK1/2 in signal transduction pathways. Finally, the results show that the protein expression of p-ERK1/2 and nuclear translocation were suppressed in D4681 group.

Key words: Taurine, Myocardial fibrosis, Proliferation, Signal transduction, Cardiac remodeling

WANG Li-ying, LI Hong, YANG Shi-jie. Taurine Inhibits Myocardial Fibrosis via PKC-ERK1/2 Signaling Pathways[J]. Chemical Research in Chinese Universities, 2012, 28(1): 84-90.

References

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Taurine Inhibits Myocardial Fibrosis via PKC-ERK1/2 Signaling Pathways

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