CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (6): 996-999.

• Articles • Previous Articles     Next Articles

Denaturation Kinetics of N-Acetyl-β-D-glucosaminidase from Scylla serrata in Urea Solution

LIN Jian-cheng1,2, LI Hua-liang1, CHEN Liang-hua1, YANG Xue-min3, WANG qin1 and CHEN Qing-xi1*   

  1. 1. Key Laboratory of Coastal and Wetland Ecosystems, Ministry of Education, School of Life Sciences, Xiamen University, Xiamen 361005, P. R. China;
    2. Department of Blood Transfusion, Xiamen Maternity and Child Health Hospital, Xiamen 361003, P. R. China;
    3. Department of Food and Biological Engineering, Zhangzhou Institute of Technical, Zhangzhou 363000, P. R. China
  • Received:2011-03-25 Revised:2011-05-20 Online:2011-11-25 Published:2011-11-07
  • Contact: CHEN Qing-xi E-mail:chenqx@xmu.edu.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.40576066) and the Science and Technology Foundation of Xiamen, China(No.3502Z20081143).

PDF (PC)

Abstract: N-Acetyl-β-D-glucosaminidase(NAGase, EC 3.2.1.52), which catalyzes the cleavage of N-acetylgluco- samine polymers, plays important roles in the molting, digestion of chitinous foods in green crab. In the study, the efforts of urea on the activity of NAGase purified from the viscera of green crab(Scylla serrata) have been studied. The results show that appropriate concentrations of urea can lead to reversible inactivation of the enzyme, and the value of the inhibitor concentration leading to 50% of enzyme activity lost(IC50) is estimated to be 0.63 mol/L. The inactivation kinetics has been studied via the kinetic method of the substrate reaction. The rate constants of inactivation have been determined. The value of k+0 is larger than that of k′+0, indicating the free enzyme molecule is more fragile than the enzyme-substrate complex in urea solution. It is suggested that the presence of the substrate offers the marked protection of this enzyme against inactivation by urea.

Key words: Green crab(Scylla serrata), N-Acetyl-β-D-glucosaminidase, Urea, Reversible inactivation kinetics