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Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (5): 773-776.

• Articles • Previous Articles     Next Articles

Studies on Fragmentation of Peptide by Nano-electrospray Ionization Fourier Transform Ion Cyclotron Resonance Multi-stage Tandem Mass Spectrometry

SHI Ying1,3, LIU Ning2, SONG Feng-rui1, LIU Zhi-qiang1* and LIU Shu-ying1   

  1. 1. Changchun Centre of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China;
    2. Central Laboratory, the Second Hospital of Jilin University, Changchun 130041, P. R. China;
    3. Graduate School of the Chinese Academy of Sciences, Beijing 100049, P. R. China
  • Received:2010-09-19 Revised:2010-11-18 Online:2011-09-25 Published:2011-09-06
  • Contact: LIU Zhi-qiang E-mail:liuzq@ciac.jl.cn
  • Supported by:

    Supported by the National Natural Science Foundation of China(No.20675079).

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Abstract: The sequence analysis of peptides was performed by nano-electrospray ionization Fourier transform ion cyclotron resonance tandem mass spectrometry(Nano-ESI-FT-ICR-MSn) and several peptides were chosen as examples. With the aid of the collision induced dissociation(CID), FT-ICR provides not only precise mass/charge ratio, but also structure information of the selected peptides. The fragment ions were identified according to the observed molecular weights and peptide sequence was determined successfully. So Nano-ESI-FT-ICR-MSn is a useful tool for identification of the amino acid sequence of peptides with high confidence. Besides, a pathway for the dehydration of y ions without amino acids containing carboxylic acid under sustained off-resonance irradiation collision-induced dissociation(SORI-CID) condition was proposed.

Key words: Nano-electrospray ionization Fourier transform ion cyclotron resonance tandem mass spectrometry , (Nano-ESI-FT-ICR-MS), Collision induced dissociation(CID), Peptide sequence