CRCU

Chemical Research in Chinese Universities ›› 2011, Vol. 27 ›› Issue (4): 550-556.

• Articles • Previous Articles     Next Articles

Comparison of Different Cartridges of Solid Phase Extraction for Determination of Polyphenols in Tobacco by UPLC/MS/MS and Multivariate Analysis

ZHANG Xia1*, LIU Wei1, XU Yong1, YANG Liu2, KONG Wei-song1, RUI Xiao-dong1, YANG Shuai1, CHEN Yong-kuan1 and MIAO Ming-ming1   

  1. 1. Key Laboratory of Tobacco Chemistry, Yunnan Academy of Tobacco Science, Kunming 650106, P. R. China;
    2. Research and Development Center of Hongta Tobacco(Group) Corporation Limited, Yuxi 653100, P. R. China
  • Received:2011-02-13 Revised:2011-06-20 Online:2011-07-25 Published:2011-06-29
  • Contact: ZHANG Xia E-mail:zhangxia840511@163.com
  • Supported by:

    Supported by the Foundation of State Tabacco Monopoly Bureau(China) in Research Project(No.110200902008).

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Abstract: The comparison of solid phase extraction(SPE) for the preconcentration and isolation of polyphenols in tobacco samples was carried out by ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC/MS/MS) and multivariate analysis. Several adsorbing materials of SPE(C18, NH2, SAX and OASIS) were investigated. It was found that the C18 and OASIS cartridges can not only speed up the purification process, but also simplify the SPE operation. A UPLC/MS/MS was used for the determination of polyphenols in tobacco samples after purification. All analytes were separated and determined in 2 min. The limit of detection was 0.05 ng/mL. Cluster analysis(CA) and principal component analysis(PCA) were used for the analysis of 4 varieties(flue-cured tobacco, oriental tobacco, sun-cured tobacco and burley) in order to interpret the effect of planting and machining process on the concentration of polyphenols. The different types of tobacco samples could be easily clustered by CA. PCA on the chemical composition of tobacco resulted in two principal components(PCs) that take 84.2% of the total variance. The PCA and CA indicate that the polyphenols can be used for distinguishing tobacco types.

Key words: Solid phase extraction, Polyphenol, Ultra-high performance liquid chromatography/tandem mass spectrometry(UPLC/MS/MS), Tobacco, Cluster analysis, Principal component analysis